1go7: Difference between revisions

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[[Image:1go7.jpg|left|200px]]


{{Structure
==The metzincin's methionine: PrtC M226C-E189K double mutant==
|PDB= 1go7 |SIZE=350|CAPTION= <scene name='initialview01'>1go7</scene>, resolution 2.10&Aring;
<StructureSection load='1go7' size='340' side='right'caption='[[1go7]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
|SITE= <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+P'>AC1</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
<table><tr><td colspan='2'>[[1go7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GO7 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1go7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1go7 OCA], [https://pdbe.org/1go7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1go7 RCSB], [https://www.ebi.ac.uk/pdbsum/1go7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1go7 ProSAT]</span></td></tr>
 
</table>
'''THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT'''
== Function ==
 
[https://www.uniprot.org/uniprot/PRTC_DICCH PRTC_DICCH]  
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1go7_consurf.spt"</scriptWhenChecked>
1GO7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA].
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11445573 11445573]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1go7 ConSurf].
[[Category: Erwinia chrysanthemi]]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Hege, T.]]
</StructureSection>
[[Category: CA]]
[[Category: Dickeya chrysanthemi]]
[[Category: PO4]]
[[Category: Large Structures]]
[[Category: ZN]]
[[Category: Hege T]]
[[Category: hydrolase]]
[[Category: metalloprotease]]
[[Category: protease]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:25:45 2008''

Latest revision as of 11:23, 27 March 2024

The metzincin's methionine: PrtC M226C-E189K double mutant

1go7, resolution 2.10Å

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