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[[Image:1god.gif|left|200px]]


{{Structure
==MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI==
|PDB= 1god |SIZE=350|CAPTION= <scene name='initialview01'>1god</scene>, resolution 2.8&Aring;
<StructureSection load='1god' size='340' side='right'caption='[[1god]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1god]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerrophidion_godmani Cerrophidion godmani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GOD FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1god FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1god OCA], [https://pdbe.org/1god PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1god RCSB], [https://www.ebi.ac.uk/pdbsum/1god PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1god ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA2H2_CERGO PA2H2_CERGO] Snake venom phospholipase A2 (PLA2) that lacks enzymatic activity and shows myotoxicity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/go/1god_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1god ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.


'''MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI'''
Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani.,Arni RK, Fontes MR, Barberato C, Gutierrez JM, Diaz C, Ward RJ Arch Biochem Biophys. 1999 Jun 15;366(2):177-82. PMID:10356281<ref>PMID:10356281</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1god" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
Lys49-Phospholipase A2 (Lys49-PLA2) homologues damage membranes by a Ca2+-independent mechanism which does not involve catalytic activity. With the aim of determining the structural basis for this novel activity, we have solved the crystal structure of myotoxin-II, a Lys49-PLA2 isolated from the venom of Cerrophidion (Bothrops) godmani (godMT-II) at 2.8 A resolution by molecular replacement. The final model has been refined to a final crystallografic residual (Rfactor) of 18.8% (Rfree = 28.2%), with excellent stereochemistry. godMT-II is also monomeric in the crystalline state, and small-angle X-ray scattering results demonstrate that the protein is monomeric in solution under fisicochemical conditions similar to those used in the crystallographic studies.
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
 
== References ==
==About this Structure==
<references/>
1GOD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cerrophidion_godmani Cerrophidion godmani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GOD OCA].
__TOC__
 
</StructureSection>
==Reference==
Crystal structure of myotoxin II, a monomeric Lys49-phospholipase A2 homologue isolated from the venom of Cerrophidion (Bothrops) godmani., Arni RK, Fontes MR, Barberato C, Gutierrez JM, Diaz C, Ward RJ, Arch Biochem Biophys. 1999 Jun 15;366(2):177-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10356281 10356281]
[[Category: Cerrophidion godmani]]
[[Category: Cerrophidion godmani]]
[[Category: Phospholipase A(2)]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Arni RK]]
[[Category: Arni, R K.]]
[[Category: Barberato C]]
[[Category: Barberato, C.]]
[[Category: Diaz-Oreiro C]]
[[Category: Diaz-Oreiro, C.]]
[[Category: Fontes MRM]]
[[Category: Fontes, M R.M.]]
[[Category: Gutierrez JM]]
[[Category: Gutierrez, J M.]]
[[Category: Ward RJ]]
[[Category: Ward, R J.]]
[[Category: bothrop]]
[[Category: lys49-phospholipase a2]]
[[Category: snake venom]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:25:48 2008''

Latest revision as of 07:16, 9 October 2024

MONOMERIC LYS-49 PHOSPHOLIPASE A2 HOMOLOGUE ISOLATED FROM THE VENOM OF CERROPHIDION (BOTHROPS) GODMANI

1god, resolution 2.80Å

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