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| [[Image:1hpn.jpg|left|200px]]
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| {{Structure
| | ==N.M.R. AND MOLECULAR-MODELLING STUDIES OF THE SOLUTION CONFORMATION OF HEPARIN== |
| |PDB= 1hpn |SIZE=350|CAPTION= <scene name='initialview01'>1hpn</scene>
| | <StructureSection load='1hpn' size='340' side='right'caption='[[1hpn]]' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HPN FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hpn OCA], [https://pdbe.org/1hpn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hpn RCSB], [https://www.ebi.ac.uk/pdbsum/1hpn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hpn ProSAT]</span></td></tr> |
| |GENE=
| | </table> |
| }}
| | __TOC__ |
| | | </StructureSection> |
| '''N.M.R. AND MOLECULAR-MODELLING STUDIES OF THE SOLUTION CONFORMATION OF HEPARIN'''
| | [[Category: Large Structures]] |
| | | [[Category: Forster MJ]] |
| | | [[Category: Mulloy B]] |
| ==Overview== | |
| The solution conformations of heparin and de-N-sulphated, re-N-acetylated heparin have been determined by a combination of n.m.r. spectroscopic and molecular-modelling techniques. The 1H- and 13C-n.m.r. spectra of these polysaccharides have been assigned. Observed 1H-1H nuclear Overhauser enhancements (n.O.e.s) have been simulated using the program NOEMOL [Forster, Jones and Mulloy (1989) J. Mol. Graph. 7, 196-201] for molecular models derived from conformational-energy calculations; correlation times for the simulations were chosen to fit experimentally determined 13C spin-lattice relaxation times. In order to achieve good agreement between calculated and observed 1H-1H n.O.e.s it was necessary to assume that the reorientational motion of the polysaccharide molecules was not isotropic, but was that of a symmetric top. The resulting model of heparin in solution is similar to that determined in the fibrous state by X-ray-diffraction techniques [Nieduszynski, Gardner and Atkins (1977) Am. Chem. Soc. Symp. Ser. 48, 73-80].
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| ==About this Structure== | |
| 1HPN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HPN OCA].
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| ==Reference==
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| N.m.r. and molecular-modelling studies of the solution conformation of heparin., Mulloy B, Forster MJ, Jones C, Davies DB, Biochem J. 1993 Aug 1;293 ( Pt 3):849-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8352752 8352752]
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| [[Category: Protein complex]] | |
| [[Category: Forster, M J.]] | |
| [[Category: Mulloy, B.]] | |
| [[Category: glycosaminoglycan]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:40:02 2008''
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