1ij6: Difference between revisions

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[[Image:1ij6.gif|left|200px]]


{{Structure
==CA2+-BOUND STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD==
|PDB= 1ij6 |SIZE=350|CAPTION= <scene name='initialview01'>1ij6</scene>, resolution 3.1&Aring;
<StructureSection load='1ij6' size='340' side='right'caption='[[1ij6]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
<table><tr><td colspan='2'>[[1ij6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IJ6 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
|GENE=
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ij6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ij6 OCA], [https://pdbe.org/1ij6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ij6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ij6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ij6 ProSAT]</span></td></tr>
 
</table>
'''CA2+-BOUND STRUCTURE OF MULTIDOMAIN EF-HAND PROTEIN, CBP40, FROM TRUE SLIME MOLD'''
== Function ==
 
[https://www.uniprot.org/uniprot/LAV1_PHYPO LAV1_PHYPO]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Acellular slime mold, Physarum polycephalum, has a unique wound-healing system. When cytoplasm of plasmodia is exposed to extracellular fluid, calcium binding protein 40 (CBP40) seals damaged areas, forming large aggregates Ca(2+) dependently. Part of the CBP40 is truncated at the N terminus by a proteinase in plasmodia (CBP40delta), which does not aggregate in the Ca(2+)-bound form. Here we report the crystal structures of CBP40delta in both the metal-free and the Ca(2+)-bound states. Both structures consist of three domains: coiled-coil, intervening, and EF-hand. The topology of the EF-hand domain is similar to that of calpain. The N-terminal half of CBP40Delta interacts with the C-terminal EF-hands through a large hydrophobic interface, necessary for high Ca(2+) affinity. Conformational change upon Ca(2+) binding is small; however, the structure of CBP40delta provides novel insights into the mechanism of Ca(2+)-dependent oligomerization.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ij/1ij6_consurf.spt"</scriptWhenChecked>
1IJ6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ6 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Metal-free and Ca2+-bound structures of a multidomain EF-hand protein, CBP40, from the lower eukaryote Physarum polycephalum., Iwasaki W, Sasaki H, Nakamura A, Kohama K, Tanokura M, Structure. 2003 Jan;11(1):75-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12517342 12517342]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ij6 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Physarum polycephalum]]
[[Category: Physarum polycephalum]]
[[Category: Single protein]]
[[Category: Iwasaki W]]
[[Category: Iwasaki, W.]]
[[Category: Kohama K]]
[[Category: Kohama, K.]]
[[Category: Nakamura A]]
[[Category: Nakamura, A.]]
[[Category: Sasaki H]]
[[Category: Sasaki, H.]]
[[Category: Tanokura M]]
[[Category: Tanokura, M.]]
[[Category: CA]]
[[Category: cbp40]]
[[Category: fourty kda calcium binding protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:51:01 2008''
Retrieved from "https://proteopedia.org/1ij6"