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[[Image:1jlv.gif|left|200px]]


{{Structure
==Anopheles dirus species B glutathione S-transferases 1-3==
|PDB= 1jlv |SIZE=350|CAPTION= <scene name='initialview01'>1jlv</scene>, resolution 1.75&Aring;
<StructureSection load='1jlv' size='340' side='right'caption='[[1jlv]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene>
<table><tr><td colspan='2'>[[1jlv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_cracens Anopheles cracens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JLV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JLV FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jlv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jlv OCA], [https://pdbe.org/1jlv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jlv RCSB], [https://www.ebi.ac.uk/pdbsum/1jlv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jlv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q7KIF2_9DIPT Q7KIF2_9DIPT]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jlv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jlv ConSurf].
<div style="clear:both"></div>


'''Anopheles dirus species B glutathione S-transferases 1-3'''
==See Also==
 
*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Glutathione S-transferases (GSTs) are dimeric proteins that play an important role in cellular detoxification. Four GSTs from the mosquito Anopheles dirus species B (Ad), an important malaria vector in South East Asia, are produced by alternate splicing of a single transcription product and were previously shown to have detoxifying activity towards pesticides such as DDT. We have determined the crystal structures for two of these alternatively spliced proteins, AdGST1-3 (complexed with glutathione) and AdGST1-4 (apo form), at 1.75 and 2.45 A resolution, respectively. These GST isozymes show differences from the related GST from the Australian sheep blowfly Lucilia cuprina; in particular, the presence of a C-terminal helix forming part of the active site. This helix causes the active site of the Anopheles GSTs to be enclosed. The glutathione-binding helix alpha2 and flanking residues are disordered in the AdGST1-4 (apo) structure, yet ordered in the AdGST1-3 (GSH-bound) structure, suggesting that insect GSTs operate with an induced fit mechanism similar to that found in the plant phi- and human pi-class GSTs. Despite the high overall sequence identities, the active site residues of AdGST1-4 and AdGST1-3 have different conformations.
 
==About this Structure==
1JLV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Anopheles_cracens Anopheles cracens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JLV OCA].
 
==Reference==
The crystal structures of glutathione S-transferases isozymes 1-3 and 1-4 from Anopheles dirus species B., Oakley AJ, Harnnoi T, Udomsinprasert R, Jirajaroenrat K, Ketterman AJ, Wilce MC, Protein Sci. 2001 Nov;10(11):2176-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11604524 11604524]
[[Category: Anopheles cracens]]
[[Category: Anopheles cracens]]
[[Category: Glutathione transferase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Harnnoi T]]
[[Category: Harnnoi, T.]]
[[Category: Jirajaroenrat K]]
[[Category: Jirajaroenrat, K.]]
[[Category: Ketterman AJ]]
[[Category: Ketterman, A J.]]
[[Category: Oakley AJ]]
[[Category: Oakley, A J.]]
[[Category: Udomsinprasert R]]
[[Category: Udomsinprasert, R.]]
[[Category: Wilce MC]]
[[Category: Wilce, M C.]]
[[Category: GSH]]
[[Category: adgst1-3]]
[[Category: glutathione s-transferase]]
[[Category: gst]]
 
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Retrieved from "https://proteopedia.org/1jlv"