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==The structure of a bacterial homing endonuclease : I-Ssp6803I==
==The structure of a bacterial homing endonuclease : I-Ssp6803I==
<StructureSection load='2ost' size='340' side='right' caption='[[2ost]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='2ost' size='340' side='right'caption='[[2ost]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OST FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ost]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803 Synechocystis sp. PCC 6803]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OST OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OST FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [http://www.ebi.ac.uk/pdbsum/2ost PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ost FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ost OCA], [https://pdbe.org/2ost PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ost RCSB], [https://www.ebi.ac.uk/pdbsum/2ost PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ost ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q57253_SYNY3 Q57253_SYNY3]
The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
 
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif.,Zhao L, Bonocora RP, Shub DA, Stoddard BL EMBO J. 2007 May 2;26(9):2432-42. Epub 2007 Apr 5. PMID:17410205<ref>PMID:17410205</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Synechocystis sp.]]
[[Category: Large Structures]]
[[Category: Bonocora, R P.]]
[[Category: Synechocystis sp. PCC 6803]]
[[Category: Shub, D A.]]
[[Category: Bonocora RP]]
[[Category: Stoddard, B L.]]
[[Category: Shub DA]]
[[Category: Zhao, L.]]
[[Category: Stoddard BL]]
[[Category: Group i intron]]
[[Category: Zhao L]]
[[Category: Homing endonuclease]]
[[Category: Hydrolase-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Restriction enzyme fold]]
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