4v2k: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 4v2k is ON HOLD Authors: Grabarczyk, D.B., Chappell, P.E., Eisel, B., Johnson, S., Lea, S.M., Berks, B.C. Description: Crystal structure of the thi... |
No edit summary |
||
| (7 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Crystal structure of the thiosulfate dehydrogenase TsdA in complex with thiosulfate== | ||
<StructureSection load='4v2k' size='340' side='right'caption='[[4v2k]], [[Resolution|resolution]] 1.29Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4v2k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Allochromatium_vinosum Allochromatium vinosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V2K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V2K FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.29Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=THJ:THIOSULFATE'>THJ</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v2k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v2k OCA], [https://pdbe.org/4v2k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v2k RCSB], [https://www.ebi.ac.uk/pdbsum/4v2k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v2k ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/TSDA_ALLVD TSDA_ALLVD] Catalyzes the oxidation of 2 molecules of thiosulfate to tetrathionate.<ref>PMID:16995898</ref> <ref>PMID:22779704</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Thiosulfate dehydrogenase (TsdA) catalyzes the oxidation of two thiosulfate molecules to form tetrathionate and is predicted to use an unusual cysteine-ligated heme as the catalytic cofactor. We have determined the structure of Allochromatium vinosum TsdA to a resolution of 1.3 A. This structure confirms the active site heme ligation, identifies a thiosulfate binding site within the active site cavity, and reveals an electron transfer route from the catalytic heme, through a second heme group to the external electron acceptor. We provide multiple lines of evidence that the catalytic reaction proceeds through the intermediate formation of a S-thiosulfonate derivative of the heme cysteine ligand: the cysteine is reactive and is accessible to electrophilic attack; cysteine S-thiosulfonate is formed by the addition of thiosulfate or following the reverse reaction with tetrathionate; the S-thiosulfonate modification is removed through catalysis; and alkylating the cysteine blocks activity. Active site amino acid residues required for catalysis were identified by mutagenesis and are inferred to also play a role in stabilizing the S-thiosulfonate intermediate. The enzyme SoxAX, which catalyzes the first step in the bacterial Sox thiosulfate oxidation pathway, is homologous to TsdA and can be inferred to use a related catalytic mechanism. | |||
Mechanism of thiosulfate oxidation in the SoxA family of cysteine-ligated cytochromes.,Grabarczyk DB, Chappell PE, Eisel B, Johnson S, Lea SM, Berks BC J Biol Chem. 2015 Apr 3;290(14):9209-21. doi: 10.1074/jbc.M114.618025. Epub 2015 , Feb 11. PMID:25673696<ref>PMID:25673696</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4v2k" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Allochromatium vinosum]] | |||
[[Category: Large Structures]] | |||
[[Category: Berks BC]] | |||
[[Category: Chappell PE]] | |||
[[Category: Eisel B]] | |||
[[Category: Grabarczyk DB]] | |||
[[Category: Johnson S]] | |||
[[Category: Lea SM]] | |||
Latest revision as of 10:39, 10 January 2024
Crystal structure of the thiosulfate dehydrogenase TsdA in complex with thiosulfate
| ||||||||||||
