4v3b: Difference between revisions
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The | ==The structure of alpha2,3-sialyltransferase variant 1 from Pasteurella dagmatis in complex with the donor product CMP== | ||
<StructureSection load='4v3b' size='340' side='right'caption='[[4v3b]], [[Resolution|resolution]] 2.18Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4v3b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_dagmatis Pasteurella dagmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V3B FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.18Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v3b OCA], [https://pdbe.org/4v3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v3b RCSB], [https://www.ebi.ac.uk/pdbsum/4v3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v3b ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/K9UUI6_9PAST K9UUI6_9PAST] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Structure-guided active-site redesign of a family GT-80 beta-D-galactoside sialyltransferase (from Pasteurella dagmatis) to change enzyme regioselectivity from alpha-2,3 in the wild type to alpha-2,6 in a P7H-M117A double mutant is reported. Biochemical data for sialylation of lactose together with protein crystal structures demonstrate highly precise enzyme engineering. | |||
Complete switch from alpha-2,3- to alpha-2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign.,Schmolzer K, Czabany T, Luley-Goedl C, Pavkov-Keller T, Ribitsch D, Schwab H, Gruber K, Weber H, Nidetzky B Chem Commun (Camb). 2015 Feb 21;51(15):3083-6. doi: 10.1039/c4cc09772f. PMID:25619424<ref>PMID:25619424</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4v3b" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pasteurella dagmatis]] | |||
[[Category: Czabany T]] | |||
[[Category: Gruber K]] | |||
[[Category: Luley-Goedl C]] | |||
[[Category: Nidetzky B]] | |||
[[Category: Pavkov-Keller T]] | |||
[[Category: Ribitsch D]] | |||
[[Category: Schmoelzer K]] | |||
[[Category: Schwab H]] | |||
Latest revision as of 10:39, 10 January 2024
The structure of alpha2,3-sialyltransferase variant 1 from Pasteurella dagmatis in complex with the donor product CMP
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