3aq4: Difference between revisions

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==Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b==
==Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b==
<StructureSection load='3aq4' size='340' side='right' caption='[[3aq4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='3aq4' size='340' side='right'caption='[[3aq4]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3aq4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AQ4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3aq4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AQ4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3aq2|3aq2]], [[3aq3|3aq3]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aq4 OCA], [https://pdbe.org/3aq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aq4 RCSB], [https://www.ebi.ac.uk/pdbsum/3aq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aq4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3aq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aq4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3aq4 RCSB], [http://www.ebi.ac.uk/pdbsum/3aq4 PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/ARF1_ARATH ARF1_ARATH] GTP-binding protein involved in protein trafficking; required for the sequence-specific vacuolar sorting route to the lytic vacuole, for the ER-to-Golgi transport and for the Golgi-derived transport to the plasma membrane. Involved in the recruitment of COPI and GDAP1 to membranes.<ref>PMID:12177464</ref> <ref>PMID:12182707</ref> <ref>PMID:12724547</ref> <ref>PMID:15659621</ref> <ref>PMID:17307898</ref>  
The Agrobacterium Ti plasmid (T-DNA) 6b proteins interact with many different host proteins implicated in plant cell proliferation. Here, we show that Arabidopsis plants overexpressing 6b display microRNA (miRNA) deficiency by directly targeting SERRATE and AGO1 via a specific loop fragment (residues 40-55). In addition, we report the crystal structures of Agrobacterium tumefaciens AK6b at 2.1 A, Agrobacterium vitis AB6b at 1.65 A, and Arabidopsis ADP ribosylation factor (ARF) at 1.8 A. The 6b structure adopts an ADP-ribosylating toxin fold closely related to cholera toxin. In vitro ADP ribosylation analysis demonstrates that 6b represents a new toxin family, with Tyr 66, Thr 93, and Tyr 153 as the ADP ribosylation catalytic residues in the presence of Arabidopsis ARF and GTP. Our work provides molecular insights, suggesting that 6b regulates plant cell growth by the disturbance of the miRNA pathway through its ADP ribosylation activity.
 
Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b.,Wang M, Soyano T, Machida S, Yang JY, Jung C, Chua NH, Yuan YA Genes Dev. 2011 Jan 1;25(1):64-76. Epub 2010 Dec 14. PMID:21156810<ref>PMID:21156810</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Wang, M M.]]
[[Category: Large Structures]]
[[Category: Yuan, Y A.]]
[[Category: Wang MM]]
[[Category: Adp-ribosylation]]
[[Category: Yuan YA]]
[[Category: Adp-ribosylation factor]]
[[Category: Mirna processing machinery]]
[[Category: Toxin]]

Latest revision as of 14:01, 13 March 2024

Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b

3aq4, resolution 1.80Å

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