4wwu: Difference between revisions
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New page: '''Unreleased structure''' The entry 4wwu is ON HOLD until sometime in the future Authors: Aibara, S., Valkov, E., Stewart, M. Description: Structure of Mex67:Mtr2 |
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The | ==Structure of Mex67:Mtr2== | ||
<StructureSection load='4wwu' size='340' side='right'caption='[[4wwu]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4wwu]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.301Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwu OCA], [https://pdbe.org/4wwu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwu RCSB], [https://www.ebi.ac.uk/pdbsum/4wwu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The Mex67:Mtr2 complex is the principal yeast nuclear export factor for bulk mRNA and also contributes to ribosomal subunit export. Mex67 is a modular protein constructed from four domains (RRM, LRR, NTF2-like and UBA) that have been thought to be joined by flexible linkers like beads on a string, with the RRM and LRR domains binding RNAs and the NTF2-like and UBA domains binding FG-nucleoporins to facilitate movement through nuclear pores. Here, we show that the NTF2-like domain from Saccharomyces cerevisiae Mex67:Mtr2 also contributes to RNA binding. Moreover, the 3.3 A resolution crystal structure of the Mex67DeltaUBA:Mtr2 complex, supplemented with small angle X-ray scattering data, indicated that the LRR domain has a defined spatial relationship to the Mex67NTF2L:Mtr2 region. Conversely, the RRM domain and especially the UBA domain are more mobile. The conformation assumed by the LRR and NTF2-like domains results in clusters of positively-charged residues on each becoming arranged to form a continuous interface for binding RNA on the opposite side of the complex to the region that interacts with FG-nucleoporins to facilitate passage through nuclear pores. | |||
Domain organization within the nuclear export factor Mex67:Mtr2 generates an extended mRNA binding surface.,Aibara S, Valkov E, Lamers M, Stewart M Nucleic Acids Res. 2015 Jan 23. pii: gkv030. PMID:25618852<ref>PMID:25618852</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4wwu" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae S288C]] | |||
[[Category: Aibara S]] | |||
[[Category: Stewart M]] | |||
[[Category: Valkov E]] | |||
