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==Crystal structure of Influenza B polymerase with bound vRNA promoter (form FluB2)==
==Crystal structure of Influenza B polymerase with bound vRNA promoter (form FluB2)==
<StructureSection load='4wrt' size='340' side='right' caption='[[4wrt]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
<StructureSection load='4wrt' size='340' side='right'caption='[[4wrt]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4wrt]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WRT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WRT FirstGlance]. <br>
<table><tr><td colspan='2'>[[4wrt]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Influenza_B_virus Influenza B virus] and [https://en.wikipedia.org/wiki/Influenza_B_virus_(B/Memphis/13/2003) Influenza B virus (B/Memphis/13/2003)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WRT FirstGlance]. <br>
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wrt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wrt RCSB], [http://www.ebi.ac.uk/pdbsum/4wrt PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wrt OCA], [https://pdbe.org/4wrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wrt RCSB], [https://www.ebi.ac.uk/pdbsum/4wrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wrt ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5V8Y6_9INFB Q5V8Y6_9INFB]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Influenza virus polymerase uses a capped primer, derived by 'cap-snatching' from host pre-messenger RNA, to transcribe its RNA genome into mRNA and a stuttering mechanism to generate the poly(A) tail. By contrast, genome replication is unprimed and generates exact full-length copies of the template. Here we use crystal structures of bat influenza A and human influenza B polymerases (FluA and FluB), bound to the viral RNA promoter, to give mechanistic insight into these distinct processes. In the FluA structure, a loop analogous to the priming loop of flavivirus polymerases suggests that influenza could initiate unprimed template replication by a similar mechanism. Comparing the FluA and FluB structures suggests that cap-snatching involves in situ rotation of the PB2 cap-binding domain to direct the capped primer first towards the endonuclease and then into the polymerase active site. The polymerase probably undergoes considerable conformational changes to convert the observed pre-initiation state into the active initiation and elongation states.
Structural insight into cap-snatching and RNA synthesis by influenza polymerase.,Reich S, Guilligay D, Pflug A, Malet H, Berger I, Crepin T, Hart D, Lunardi T, Nanao M, Ruigrok RW, Cusack S Nature. 2014 Nov 19. doi: 10.1038/nature14009. PMID:25409151<ref>PMID:25409151</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wrt" style="background-color:#fffaf0;"></div>
==See Also==
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: RNA-directed RNA polymerase]]
[[Category: Influenza B virus]]
[[Category: Cusack, S]]
[[Category: Large Structures]]
[[Category: Guilligay, D]]
[[Category: Cusack S]]
[[Category: Pflug, A]]
[[Category: Guilligay D]]
[[Category: Reich, S]]
[[Category: Pflug A]]
[[Category: Transferase-rna complex]]
[[Category: Reich S]]
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