Chitinase: Difference between revisions

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~~{{STRUCTURE_1edq| PDB~~=~~1edq | SIZE~~=~~400| SCENE~~=~~Chitinases/Cv/2 |~~right~~|CAPTION~~=Chitinase ~~I from ''Serratia marcescens'',~~ [[~~1edq~~]] }}

__TOC__

== Function ==

[[Chitinase]]s cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium ([[1ctn]]) and a plant ([[2hvm]]). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed.

[[Chitinase]]s (Chi) cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium ([[1ctn]]) and a plant ([[2hvm]]). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed. <ref>PMID:21054166</ref>.

~~== 3D Structures of~~ Chitinase ==

* '''Chitinase I''' is found in plants.

* '''Chitinase II''' is found in plants, fungi and bacteria.

* '''Chitinase III''' has no sequence similarity to class I or II.

* '''Chitinase IV''' has similar characteristics to class I but are smaller than it.

~~Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~

==Disease ==

~~{{#tree:id~~=~~OrganizedByTopic|openlevels~~=0|

*Chitinase I

Chi is related to allergies and asthma<ref>PMID:19295241</ref>.

**[[3aro]], [[3b8s]] – VhChi residues 22-597 – ''Vibrio harveyi''

== Structural highlights ==

**[[3b9e]] - VhChi residues 22-597 (mutant)

**[[1k85]] – BcChi fibronectin domain – ''Bacillus circulans'' – NMR

**[[1itx]] - BcChi catalytic domain

**[[1ed7]] – BcChi chitin-binding domain - NMR

**[[1ll6]], [[1ll7]] - CiChi residues 36-427 (mutant) – ''Coccicioides immitis''

**[[1d2k]] - CiChi residues 36-427

**[[3n11]] – BcChi – ''Bacillus cereus''

**[[3iwr]], [[2dkv]] – Chi residues 33-340 – Japanese rice

**[[1edq]], [[1ctn]], [[4axn]] - SmChi – ''Serratia marcescens''

**[[1x6l]], [[1rd6]] – SmChi (mutant)

**[[3g6l]] - BoChi residues 21-426 – ''Bionectria ochroleuca''

**[[3oa5]], [[4dws]] – Chi – ''Yersinia entomophaga''

**[[3w4r]] – Chi – corn borer

*''Chitinase ~~I binary complex''~~

The <scene name='27/271631/Cv/5'>chitin tetrasaccharide binding site</scene> of Chitinase A. <ref>PMID:24582745</ref> Water molecules are shown as red spheres.

**[[3arp]], [[3arq]], [[3arr]], [[3ars]], [[3art]], [[3aru]], [[3arv]], [[3arw]], [[3arx]], [[3ary]], [[3arz]] – VhChi residues 22-597 + inhibitor

== 3D Structures of Chitinase ==

**[[3as0]], [[3as1]], [[3as2]], [[3as3]] – VhChi residues 22-597 (mutant) + inhibitor

[[Chitinase 3D structures]]

**[[3b9a]], [[3b9d]] - VhChi residues 22-597 + polysaccharide

**[[2xvn]], [[2xuc]] – AfChi residues 29-307 + inhibitor – ''Aspergillus fumigatus''

**[[3n13]], [[3n15]], [[3n17]], [[3n18]] – BcChi (mutant) + NAG

**[[3n12]] – BcChi + Zn

**[[3n1a]] - BcChi (mutant) + inhibitor

**[[1x6n]] - SmChi (mutant) + inhibitor

**[[1ffq]] - SmChi + inhibitor

**[[1nh6]], [[1k9t]], [[1ffr]], [[1eib]], [[1ehn]] - SmChi (mutant) + polysaccharide

**[[2wk2]], [[2wly]], [[2wlz]], [[2wm0]] – SmChi residues 2-520 + inhibitor

**[[3g6m]] - BoChi residues 21-426 + caffeine

**[[1waw]], [[1wb0]] – hChi catalytic domain polypeptide – human

**[[1hkk]], [[1hkm]], [[3rm4]], [[3rm8]], [[3rm9]], [[3rme]] – hChi catalytic domain inhibitor

**[[1ll4]] - CiChi residues 36-427 + inhibitor

*Chitinase II

**[[1e15]], [[3wd0]] - SmChi

**[[1ogb]], [[1goi]], [[1e6p]] - SmChi (mutant)

**[[1kfw]] – Chi catalytic domain – Arthrobacter

**[[1dxj]] – Chi – Jack bean

*''Chitinase II binary complex''

**[[1ogg]], [[1ur9]] - SmChi (mutant) + inhibitor

**[[1e6n]] - SmChi (mutant) + polysaccharide

**[[1ur8]], [[1gpf]], [[1e6r]], [[3wd1]], [[3wd2]], [[3wd3]], [[3wd4]] - SmChi + inhibitor

**[[1e6z]] – SmChi + intermediate

**[[2a3a]], [[2a3b]], [[2a3c]], [[2a3e]] – AfChi + inhibitor

**[[1w1p]], [[1w1t]], [[1w1v]], [[1w1y]], [[1o6i]], [[1h0g]], [[1h0i]] – SmChi + polypeptide

*Chitinase ~~III~~

**[[~~2dbt]], [[1wvu]] – SgChi residues 30-294 – ''Streptomyces griseus'' ~~

**[[1wvv]] - SgChi residues 30-294 (mutant)

**[[2d49]] – SgChi chitin-binding domain - NMR

**[[2xvp]] – AfChi

**[[2xtk]] – AfChi + inhibitor

*Chitinase IV

**[[3hbd]], [[3hbe]], [[3hbh]] - Chi catalytic domain – Norway spruce

*Chitinase V

**[[3alf]] – tChi residues 26-377 – tobacco

*''Chitinase ~~V binary complex''~~

**[[3alg]] - tChi residues 26-377 (mutant) + NAG

**[[3chc]], [[3chd]], [[3che]], [[3chf]] - AfChi + polypeptide

**[[3ch9]], [[2iuz]] – AfChi + inhibitor

*Chitinase VIII

**[[2cjl]] - Chi residues 41-244 – ''Streptomyces coelicolor''

*Chitinase XVIII

**[[3sim]] – Chi – ''Crocus vernus''

*Chitinase C

**[[4dyg]] – RyChiC + NAG – Rye

**[[4dwx]] - RyChiC

**[[4j0l]] – RyChiC + GlcNAC

*Other chitinases

**[[1wno]], [[1w9p]] - AfChi

**[[2dsk]] - PfChi catalytic domain – ''Pyrococcus furiosus''

**[[3afb]] - PfChi catalytic domain (mutant)

**[[1cns]] – Chi – ''Hordeum vulgare''

**[[2czn]] - PfChi chitin-binding domain – NMR

**[[2cwr]] - PfChi chitin-binding domain

**[[3ian]] – Chi (mutant) – ''Lactococcus lactis''

**[[3fxy]] - hChi catalytic domain

**[[1lq0]] - hChi residues 22-286

**[[1guv]] - hChi residues 22-387

**[[3fnd]], [[3co4]] – Chi – ''Bacteroides thetaiotamicron''

**[[3cql]] – Chi – ''Carica papaya''

**[[2z37]] - BjChi catalytic domain – ''Brassica juncea''

**[[2z39]] – BjChi catalytic domain (mutant)

**[[2uy2]] – yChi residues 22-315 – yeast

**[[4hmc]] – MmChi 60 – ''Moritella marina''

**[[3w3e]] – Chi – cowpea

**[[3w6b]] – RaChi catalytic domain – ''Ralstonia''

**[[3w6e]] ~~– RaChi catalytic domain (mutant) ~~

*''Other chitinases binary complex''

</StructureSection>

**[[3a4w]], [[3a4x]] – PfChi catalytic domain (mutant) + NAG

== References ==

**[[1lg1]] - hChi residues 22-387 + chitobiose

**[[1lg2]] - hChi residues 22-387 + ethylene glycol

**[[3fy1]], [[2ybt]], [[2ybu]] - hChi catalytic domain + inhibitor

**[[1hki]], [[1hkj]] - hChi + inhibitor

**[[2z38]] - BjChi catalytic domain + Cl

**[[2uy3]], [[2uy4]], [[2uy5]] - yChi residues 22-315 + inhibitor

**[[1w9v]], [[1w9u]] - AfChi + polypeptide

**[[4hmd]] – MmChi 60 + intermediate

**[[4hme]] – MmChi 60 + NAG

**[[3w6c]] – RaChi catalytic domain + disaccharide

**[[3w6d]], [[3w6f]] – RaChi catalytic domain (mutant) + polysaccharide

}}

[[Category:Topic Page]]

@@ Line 1: / Line 1: @@
-{{STRUCTURE_1edq|  PDB=1edq  | SIZE=400| SCENE=Chitinases/Cv/2 |right|CAPTION=Chitinase I from ''Serratia marcescens'', [[1edq]] }}
+<StructureSection load='3wh1' size='350' side='right' caption='Chitinase A complex with (GlcNac)4 and ethylene glycol (PDB entry [[3wh1]])' scene='27/271631/Cv/3'>
+__TOC__
+== Function ==
-[[Chitinase]]s cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium ([[1ctn]]) and a plant ([[2hvm]]). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed.
+[[Chitinase]]s (Chi) cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium ([[1ctn]]) and a plant ([[2hvm]]). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed. <ref>PMID:21054166</ref>. <br />
-== 3D Structures of Chitinase ==
+* '''Chitinase I''' is found in plants.<br />
+* '''Chitinase II''' is found in plants, fungi and bacteria.<br />
+* '''Chitinase III''' has no sequence similarity to class I or II.<br />
+* '''Chitinase IV''' has similar characteristics to class I but are smaller than it.<br />
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+==Disease ==
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*Chitinase I
+Chi is related to allergies and asthma<ref>PMID:19295241</ref>.
-**[[3aro]], [[3b8s]] – VhChi residues 22-597 – ''Vibrio harveyi''<br />
+== Structural highlights ==
-**[[3b9e]] - VhChi residues 22-597 (mutant)<br />
-**[[1k85]] – BcChi fibronectin domain – ''Bacillus circulans'' – NMR<br />
-**[[1itx]] - BcChi catalytic domain<br />
-**[[1ed7]] – BcChi chitin-binding domain - NMR<br />
-**[[1ll6]], [[1ll7]] - CiChi residues 36-427 (mutant) – ''Coccicioides immitis'' <br />
-**[[1d2k]] - CiChi residues 36-427<br />
-**[[3n11]] – BcChi – ''Bacillus cereus''<br />
-**[[3iwr]], [[2dkv]] – Chi residues 33-340 – Japanese rice<br />
-**[[1edq]], [[1ctn]], [[4axn]] - SmChi – ''Serratia marcescens''<br />
-**[[1x6l]], [[1rd6]] – SmChi (mutant)<br />
-**[[3g6l]] - BoChi residues 21-426 – ''Bionectria ochroleuca''<br />
-**[[3oa5]], [[4dws]] – Chi – ''Yersinia entomophaga''<br />
-**[[3w4r]] – Chi – corn borer<br />
-*''Chitinase I binary complex''
+The <scene name='27/271631/Cv/5'>chitin tetrasaccharide binding site</scene> of Chitinase A. <ref>PMID:24582745</ref> Water molecules are shown as red spheres.
-**[[3arp]], [[3arq]], [[3arr]], [[3ars]], [[3art]], [[3aru]], [[3arv]], [[3arw]], [[3arx]], [[3ary]], [[3arz]] – VhChi residues 22-597 + inhibitor<br />
+== 3D Structures of Chitinase ==
-**[[3as0]], [[3as1]], [[3as2]], [[3as3]] – VhChi residues 22-597 (mutant) + inhibitor<br />
+[[Chitinase 3D structures]]
-**[[3b9a]], [[3b9d]] - VhChi residues 22-597 + polysaccharide<br />
-**[[2xvn]], [[2xuc]] – AfChi residues 29-307 + inhibitor – ''Aspergillus fumigatus''<br />
-**[[3n13]], [[3n15]], [[3n17]], [[3n18]] – BcChi (mutant) + NAG<br />
-**[[3n12]] – BcChi + Zn<br />
-**[[3n1a]] - BcChi (mutant) + inhibitor<br />
-**[[1x6n]] - SmChi (mutant) + inhibitor<br />
-**[[1ffq]] - SmChi + inhibitor<br />
-**[[1nh6]], [[1k9t]], [[1ffr]], [[1eib]], [[1ehn]] - SmChi (mutant) + polysaccharide<br />
-**[[2wk2]], [[2wly]], [[2wlz]], [[2wm0]] – SmChi residues 2-520 + inhibitor <br />
-**[[3g6m]] - BoChi residues 21-426 + caffeine<br />
-**[[1waw]], [[1wb0]] – hChi catalytic domain  polypeptide – human<br />
-**[[1hkk]], [[1hkm]], [[3rm4]], [[3rm8]], [[3rm9]], [[3rme]] – hChi catalytic domain  inhibitor<br />
-**[[1ll4]] - CiChi residues 36-427 + inhibitor<br />
-*Chitinase II
-**[[1e15]], [[3wd0]] - SmChi<br />
-**[[1ogb]], [[1goi]], [[1e6p]] - SmChi (mutant)<br />
-**[[1kfw]] – Chi catalytic domain – Arthrobacter<br />
-**[[1dxj]] – Chi – Jack bean<br />
-*''Chitinase II binary complex''
-**[[1ogg]], [[1ur9]] - SmChi (mutant) + inhibitor<br />
-**[[1e6n]] - SmChi (mutant) + polysaccharide<br />
-**[[1ur8]], [[1gpf]], [[1e6r]], [[3wd1]], [[3wd2]], [[3wd3]], [[3wd4]] - SmChi + inhibitor<br />
-**[[1e6z]] – SmChi + intermediate<br />
-**[[2a3a]], [[2a3b]], [[2a3c]], [[2a3e]] – AfChi + inhibitor<br />
-**[[1w1p]], [[1w1t]], [[1w1v]], [[1w1y]], [[1o6i]], [[1h0g]], [[1h0i]] – SmChi + polypeptide<br />
-*Chitinase III
-**[[2dbt]], [[1wvu]] – SgChi residues 30-294 – ''Streptomyces griseus''<br />
-**[[1wvv]] - SgChi residues 30-294 (mutant)<br />
-**[[2d49]] – SgChi chitin-binding domain - NMR<br />
-**[[2xvp]] – AfChi<br />
-**[[2xtk]] – AfChi + inhibitor
-*Chitinase IV
-**[[3hbd]], [[3hbe]], [[3hbh]] - Chi catalytic domain – Norway spruce<br />
-*Chitinase V
-**[[3alf]] – tChi residues 26-377 – tobacco<br />
-*''Chitinase V binary complex''
-**[[3alg]] - tChi residues 26-377 (mutant) + NAG<br />
-**[[3chc]], [[3chd]], [[3che]], [[3chf]] - AfChi + polypeptide<br />
-**[[3ch9]], [[2iuz]] – AfChi + inhibitor<br />
-*Chitinase VIII
-**[[2cjl]] - Chi residues 41-244 – ''Streptomyces coelicolor''<br />
-*Chitinase XVIII
-**[[3sim]] – Chi – ''Crocus vernus''
-*Chitinase C
-**[[4dyg]] – RyChiC + NAG – Rye<br />
-**[[4dwx]] - RyChiC<br />
-**[[4j0l]] – RyChiC + GlcNAC<br />
-*Other chitinases
-**[[1wno]], [[1w9p]] - AfChi<br />
-**[[2dsk]] - PfChi catalytic domain – ''Pyrococcus furiosus''<br />
-**[[3afb]] - PfChi catalytic domain (mutant)<br />
-**[[1cns]] – Chi – ''Hordeum vulgare''<br />
-**[[2czn]] - PfChi chitin-binding domain – NMR<br />
-**[[2cwr]] - PfChi chitin-binding domain<br />
-**[[3ian]] – Chi (mutant) – ''Lactococcus lactis''<br />
-**[[3fxy]] - hChi catalytic domain<br />
-**[[1lq0]] - hChi residues 22-286<br />
-**[[1guv]] - hChi residues 22-387<br />
-**[[3fnd]], [[3co4]] – Chi – ''Bacteroides thetaiotamicron''<br />
-**[[3cql]] – Chi – ''Carica papaya''<br />
-**[[2z37]] - BjChi catalytic domain – ''Brassica juncea''<br />
-**[[2z39]] – BjChi catalytic domain (mutant)<br />
-**[[2uy2]] – yChi residues 22-315 – yeast<br />
-**[[4hmc]] – MmChi 60 – ''Moritella marina''<br />
-**[[3w3e]] – Chi – cowpea<br />
-**[[3w6b]] – RaChi catalytic domain – ''Ralstonia''<br />
-**[[3w6e]] – RaChi catalytic domain (mutant) <br />
-*''Other chitinases  binary complex''
+</StructureSection>
-**[[3a4w]], [[3a4x]] – PfChi catalytic domain (mutant) + NAG<br />
+== References ==
-**[[1lg1]] - hChi residues 22-387 + chitobiose<br />
+<references/>
-**[[1lg2]] - hChi residues 22-387 + ethylene glycol<br />
-**[[3fy1]], [[2ybt]], [[2ybu]] - hChi catalytic domain + inhibitor<br />
-**[[1hki]], [[1hkj]] - hChi + inhibitor<br />
-**[[2z38]] - BjChi catalytic domain + Cl<br />
-**[[2uy3]], [[2uy4]], [[2uy5]] - yChi residues 22-315 + inhibitor<br />
-**[[1w9v]], [[1w9u]] - AfChi + polypeptide<br />
-**[[4hmd]] – MmChi 60 + intermediate<br />
-**[[4hme]] – MmChi 60 + NAG<br />
-**[[3w6c]] – RaChi catalytic domain + disaccharide<br />
-**[[3w6d]], [[3w6f]] – RaChi catalytic domain (mutant) + polysaccharide<br />
-}}
 [[Category:Topic Page]]

Chitinase: Difference between revisions

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