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[[Image:1ltl.gif|left|200px]]


{{Structure
==THE DODECAMER STRUCTURE OF MCM FROM ARCHAEAL M. THERMOAUTOTROPHICUM==
|PDB= 1ltl |SIZE=350|CAPTION= <scene name='initialview01'>1ltl</scene>, resolution 3.00&Aring;
<StructureSection load='1ltl' size='340' side='right'caption='[[1ltl]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
<table><tr><td colspan='2'>[[1ltl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LTL FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ltl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ltl OCA], [https://pdbe.org/1ltl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ltl RCSB], [https://www.ebi.ac.uk/pdbsum/1ltl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ltl ProSAT]</span></td></tr>
 
</table>
'''THE DODECAMER STRUCTURE OF MCM FROM ARCHAEAL M. THERMOAUTOTROPHICUM'''
== Function ==
 
[https://www.uniprot.org/uniprot/O27798_METTH O27798_METTH]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Eukaryotic chromosomal DNA is licensed for replication precisely once in each cell cycle. The mini-chromosome maintenance (MCM) complex plays a role in this replication licensing. We have determined the structure of a fragment of MCM from Methanobacterium thermoautotrophicum (mtMCM), a model system for eukaryotic MCM. The structure reveals a novel dodecameric architecture with a remarkably long central channel. The channel surface has an unusually high positive charge and binds DNA. We also show that the structure of the N-terminal fragment is conserved for all MCMs proteins despite highly divergent sequences, suggesting a common architecture for a similar task: gripping/remodeling DNA and regulating MCM activity. An mtMCM mutant protein equivalent to a yeast MCM5 (CDC46) protein with the bob1 mutation at its N terminus has only subtle structural changes, suggesting a Cdc7-bypass mechanism by Bob1 in yeast. Yeast bypass experiments using MCM5 mutant proteins support the hypothesis for the bypass mechanism.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lt/1ltl_consurf.spt"</scriptWhenChecked>
1LTL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTL OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
The structure and function of MCM from archaeal M. Thermoautotrophicum., Fletcher RJ, Bishop BE, Leon RP, Sclafani RA, Ogata CM, Chen XS, Nat Struct Biol. 2003 Mar;10(3):160-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12548282 12548282]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ltl ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Single protein]]
[[Category: Bishop BE]]
[[Category: Bishop, B E.]]
[[Category: Chen XS]]
[[Category: Chen, X S.]]
[[Category: Fletcher RJ]]
[[Category: Fletcher, R J.]]
[[Category: Leon RP]]
[[Category: Leon, R P.]]
[[Category: Ogata CM]]
[[Category: Ogata, C M.]]
[[Category: Sclafani RA]]
[[Category: Sclafani, R A.]]
[[Category: ZN]]
[[Category: replication]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:35:01 2008''
Retrieved from "https://proteopedia.org/1ltl"