Nitrate reductase: Difference between revisions

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~~{{STRUCTURE_2jim| PDB~~=~~2jim | SIZE~~=~~400| SCENE~~= |right~~|CAPTION~~=Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo ~~(green)~~ and molybdepterin guanine nucleotide (~~stick model),~~ [[~~2jim~~]] }}

~~<!--~~

== Function ==

~~Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.~~

'''Nitrate reductase''' (NR) catalyzes the reduction of NO3 to NO2 using NADPH<ref>PMID:13152110</ref>. NR active site contains Mo atom. Four types of NR are known:

~~Or use the four-green-boxes-button to insert scrollable text adjacent~~

'''eukaryotic assimilatory NR'''

~~to a Jmol applet. Check out the other buttons as well!~~

'''bacterial cytoplasmic assimilatory''' (NAC)

~~-->~~

'''bacterial membrane-bound respiratory''' (NAR)

'''Nitrate reductase''' (NR) catalyzes the reduction of ~~NO3~~ to ~~NO2~~ using NADPH . NR active site contains Mo atom. Four types of NR are known: ~~one~~ eukaryotic assimilatory NR ~~and 3~~ bacterial ~~NRs –~~ cytoplasmic assimilatory (NAC), membrane-bound respiratory (NAR) ~~and~~ periplasmic dissimilatory (NAP). The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.

'''bacterial periplasmic dissimilatory''' (NAP).

The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.

== Structural highlights ==

<scene name='49/490062/Cv/7'>Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3 and 2 molybdepterin guanine nucleotides</scene>. NAP active site includes a <scene name='49/490062/Cv/8'>six-coordinated Mo atom which coordinates to 2 MGD molecules and Cys140</scene>. The <scene name='49/490062/Cv/9'>NO3 molecule is guided to the active site by a funnel leading to the Mo atom</scene><ref>PMID:18327621</ref>.

</StructureSection>

==3D structures of nitrate reductase==

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**[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''

**[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''

**[[3ml1]], [[3o5a]] - NAP catalytic subunit + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''

**[[3ml1]], [[3o5a]] - NAP catalytic subunit + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''

**[[5yly]] – NR cytochrome b5 reductase domain + FAD – ''Ulva prolifera''

*Bacterial respiratory NR

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**[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''

*Eukaryotic NR

**[[1cne]] – cNR + FAD – corn

**[[1cnf]], [[2cnd]] – cNR (mutant) + FAD + ADP

}}

== References ==

[[Category:Topic Page]]

Nitrate reductase: Difference between revisions

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_2jim|  PDB=2jim  | SIZE=400| SCENE= |right|CAPTION=Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo (green) and molybdepterin guanine nucleotide (stick model), [[2jim]] }}
+<StructureSection load='' size='350' side='right' caption='Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3- and molybdepterin guanine nucleotide (PDB entry [[2jiq]])' scene='49/490062/Cv/3'>
-<!--
+== Function ==
-Please use the "3D" button above this box to insert a Jmol applet (molecule) on this page.
+'''Nitrate reductase''' (NR) catalyzes the reduction of NO<sub>3</sub> to NO<sub>2</sub> using NADPH<ref>PMID:13152110</ref>.  NR active site contains Mo atom.  Four types of NR are known:<br />
-Or use the four-green-boxes-button to insert scrollable text adjacent
+'''eukaryotic assimilatory NR'''<br />
-to a Jmol applet. Check out the other buttons as well!
+'''bacterial cytoplasmic assimilatory''' (NAC)<br />
--->
+'''bacterial membrane-bound respiratory''' (NAR)<br />
-'''Nitrate reductase''' (NR) catalyzes the reduction of NO3 to NO2 using NADPH .  NR active site contains Mo atom.  Four types of NR are known: one eukaryotic assimilatory NR and 3 bacterial NRs – cytoplasmic assimilatory (NAC), membrane-bound respiratory (NAR) and periplasmic dissimilatory (NAP).  The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
+'''bacterial periplasmic dissimilatory''' (NAP).<br />
+The cofactors of NR are Molybdopterin (MPT) in eukaryotic NR and bis-Molybdopterin guanine dinucleotide (MGD) in bacterial NR.
+== Structural highlights ==
+<scene name='49/490062/Cv/7'>Bacterial periplasmic dissimilatory nitrate reductase containing Fe4S4 cluster with Mo, NO3 and 2 molybdepterin guanine nucleotides</scene>. NAP active site includes a <scene name='49/490062/Cv/8'>six-coordinated Mo atom which coordinates to 2 MGD molecules and Cys140</scene>. The <scene name='49/490062/Cv/9'>NO3 molecule is guided to the active site by a funnel leading to the Mo atom</scene><ref>PMID:18327621</ref>.
+</StructureSection>
 ==3D structures of nitrate reductase==
@@ Line 21: / Line 27: @@
 **[[1ogy]] - NAP + heme + MGD + Mo– ''Rhodobacter sphaeroides''<br />
 **[[2nya]] - EcNAP + MGD + Mo – ''Escherichia coli''<br />
-**[[3ml1]], [[3o5a]] - NAP catalytic subunit  + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''
+**[[3ml1]], [[3o5a]] - NAP catalytic subunit  + NAPB + heme + MGD + Mo – ''Ralstonia eutropha''<br />
+**[[5yly]] – NR cytochrome b5 reductase domain + FAD – ''Ulva prolifera''<br />
 *Bacterial respiratory NR
@@ Line 36: / Line 43: @@
 **[[2bih]], [[2bii]] – NR MoCo-binding domain + MPT – ''Pichia angusta''
+*Eukaryotic NR
+**[[1cne]] – cNR + FAD – corn<br />
+**[[1cnf]], [[2cnd]] – cNR (mutant) + FAD + ADP<br />
 }}
+== References ==
+<references/>
 [[Category:Topic Page]]