1m8n: Difference between revisions

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[[Image:1m8n.gif|left|200px]]


{{Structure
==Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501==
|PDB= 1m8n |SIZE=350|CAPTION= <scene name='initialview01'>1m8n</scene>, resolution 2.45&Aring;
<StructureSection load='1m8n' size='340' side='right'caption='[[1m8n]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1m8n]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1M8N FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1m8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m8n OCA], [https://pdbe.org/1m8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1m8n RCSB], [https://www.ebi.ac.uk/pdbsum/1m8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1m8n ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9GSA6_CHOFU Q9GSA6_CHOFU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m8/1m8n_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1m8n ConSurf].
<div style="clear:both"></div>


'''Choristoneura Fumiferana (Spruce Budworm) Antifreeze Protein Isoform 501'''
==See Also==
 
*[[Antifreeze protein 3D structures|Antifreeze protein 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The insect spruce budworm (Choristoneura fumiferana)(Cf) produces a number of isoforms of its highly active antifreeze protein (CfAFP). Although most of the CfAFP isoforms are in the 9-kDa range, isoforms containing a 30- or 31-amino acid insertion have also been identified. Here we describe the functional and structural analysis of a selected long isoform, CfAFP-501. X-ray crystal structure determination reveals that the 31-amino acid insertion found in CfAFP-501 forms two additional loops within its highly regular beta-helical structure. This effectively extends the area of the two-dimensional Thr array and ice-binding surface of the protein. The larger isoform has 3 times the thermal hysteresis activity of the 9-kDa CfAFP-337. As well, a deletion of the 31-amino acid insertion within CfAFP-501 to form CfAFP-501-Delta-2-loop, results in a protein with reduced activity similar to the shorter CfAFP isoforms. Thus, the enhanced antifreeze activity of CfAFP-501 is directly correlated to the length of its beta-helical structure and hence the size of its ice-binding face.
 
==About this Structure==
1M8N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Choristoneura_fumiferana Choristoneura fumiferana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M8N OCA].
 
==Reference==
A beta-helical antifreeze protein isoform with increased activity. Structural and functional insights., Leinala EK, Davies PL, Doucet D, Tyshenko MG, Walker VK, Jia Z, J Biol Chem. 2002 Sep 6;277(36):33349-52. Epub 2002 Jun 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12105229 12105229]
[[Category: Choristoneura fumiferana]]
[[Category: Choristoneura fumiferana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Davies, P L.]]
[[Category: Davies PL]]
[[Category: Jia, Z.]]
[[Category: Jia Z]]
[[Category: Leinala, E K.]]
[[Category: Leinala EK]]
[[Category: antifreeze protein]]
[[Category: left-handed beta-helix]]
 
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Retrieved from "https://proteopedia.org/1m8n"