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[[Image:5eas.gif|left|200px]]<br />
<applet load="5eas" size="450" color="white" frame="true" align="right" spinBox="true"
caption="5eas, resolution 2.25&Aring;" />
'''5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM'''<br />


==Overview==
==5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM==
Terpene cyclases catalyze the synthesis of cyclic terpenes with 10-, 15-, and 20-carbon acyclic isoprenoid diphosphates as substrates. Plants have, been a source of these natural products by providing a homologous set of, terpene synthases. The crystal structures of 5-epi-aristolochene synthase, a sesquiterpene cyclase from tobacco, alone and complexed separately with, two farnesyl diphosphate analogs were analyzed. These structures reveal an, unexpected enzymatic mechanism for the synthesis of the bicyclic product, 5-epi-aristolochene, and provide a basis for understanding the, stereochemical selectivity displayed by other cyclases in the biosynthesis, of pharmacologically important cyclic terpenes. As such, these structures, provide templates for the engineering of novel terpene ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9295271 (full description)]]
<StructureSection load='5eas' size='340' side='right'caption='[[5eas]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[5eas]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EAS FirstGlance]. <br>
5EAS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Sites: MGA and MGB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=5EAS OCA]].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eas OCA], [https://pdbe.org/5eas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eas RCSB], [https://www.ebi.ac.uk/pdbsum/5eas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eas ProSAT]</span></td></tr>
Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase., Starks CM, Back K, Chappell J, Noel JP, Science. 1997 Sep 19;277(5333):1815-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9295271 9295271]
</table>
== Function ==
[https://www.uniprot.org/uniprot/5EAS_TOBAC 5EAS_TOBAC] Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ea/5eas_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=5eas ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
[[Category: Single protein]]
[[Category: Back K]]
[[Category: Back, K.]]
[[Category: Chappell J]]
[[Category: Chappell, J.]]
[[Category: Noel JP]]
[[Category: Noel, J.P.]]
[[Category: Starks CM]]
[[Category: Starks, C.M.]]
[[Category: MG]]
[[Category: 5-epi-aristolochene synthase]]
[[Category: isoprenoid biosynthesis]]
[[Category: isoprenoid cyclase]]
[[Category: isoprenoid synthase]]
[[Category: natural products biosynthesis]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:48:53 2007''
Retrieved from "https://proteopedia.org/5eas"