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==Refinement of RNA binding domain in human Tra2 beta protein==
==Refinement of RNA binding domain in human Tra2 beta protein==
<StructureSection load='2rrb' size='340' side='right' caption='[[2rrb]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
<StructureSection load='2rrb' size='340' side='right'caption='[[2rrb]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2rrb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RRB FirstGlance]. <br>
<table><tr><td colspan='2'>[[2rrb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RRB FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2rra|2rra]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rrb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rrb RCSB], [http://www.ebi.ac.uk/pdbsum/2rrb PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rrb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rrb OCA], [https://pdbe.org/2rrb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rrb RCSB], [https://www.ebi.ac.uk/pdbsum/2rrb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rrb ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/TRA2B_HUMAN TRA2B_HUMAN] Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre-mRNA.<ref>PMID:9546399</ref> <ref>PMID:12165565</ref> <ref>PMID:12761049</ref> <ref>PMID:15009664</ref>  
Human Transformer2-beta (hTra2-beta) is an important member of the serine/arginine-rich protein family, and contains one RNA recognition motif (RRM). It controls the alternative splicing of several pre-mRNAs, including those of the calcitonin/calcitonin gene-related peptide (CGRP), the survival motor neuron 1 (SMN1) protein and the tau protein. Accordingly, the RRM of hTra2-beta specifically binds to two types of RNA sequences [the CAA and (GAA)(2) sequences]. We determined the solution structure of the hTra2-beta RRM (spanning residues Asn110-Thr201), which not only has a canonical RRM fold, but also an unusual alignment of the aromatic amino acids on the beta-sheet surface. We then solved the complex structure of the hTra2-beta RRM with the (GAA)(2) sequence, and found that the AGAA tetra-nucleotide was specifically recognized through hydrogen-bond formation with several amino acids on the N- and C-terminal extensions, as well as stacking interactions mediated by the unusually aligned aromatic rings on the beta-sheet surface. Further NMR experiments revealed that the hTra2-beta RRM recognizes the CAA sequence when it is integrated in the stem-loop structure. This study indicates that the hTra2-beta RRM recognizes two types of RNA sequences in different RNA binding modes.
 
Structural basis for the dual RNA-recognition modes of human Tra2-beta RRM.,Tsuda K, Someya T, Kuwasako K, Takahashi M, He F, Unzai S, Inoue M, Harada T, Watanabe S, Terada T, Kobayashi N, Shirouzu M, Kigawa T, Tanaka A, Sugano S, Guntert P, Yokoyama S, Muto Y Nucleic Acids Res. 2011 Mar;39(4):1538-53. Epub 2010 Oct 5. PMID:20926394<ref>PMID:20926394</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Inoue, M]]
[[Category: Large Structures]]
[[Category: Kigawa, T]]
[[Category: Inoue M]]
[[Category: Kuwasako, K]]
[[Category: Kigawa T]]
[[Category: Muto, Y]]
[[Category: Kuwasako K]]
[[Category: Shirouzu, M]]
[[Category: Muto Y]]
[[Category: Someya, T]]
[[Category: Shirouzu M]]
[[Category: Sugano, S]]
[[Category: Someya T]]
[[Category: Takahashi, M]]
[[Category: Sugano S]]
[[Category: Terada, T]]
[[Category: Takahashi M]]
[[Category: Tsuda, K]]
[[Category: Terada T]]
[[Category: Yokoyama, S]]
[[Category: Tsuda K]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Yokoyama S]]
[[Category: Nppsfa]]
[[Category: Rbd]]
[[Category: Structural genomic]]
[[Category: Rna binding protein]]
[[Category: Rrm domain]]
[[Category: Rsgi]]

Latest revision as of 07:06, 1 May 2024

Refinement of RNA binding domain in human Tra2 beta protein

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