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==Structure of AAV-DJ, a Retargeted Gene Therapy Vector: Cryo-Electron Microscopy at 4.5A resolution==
==Structure of AAV-DJ, a Retargeted Gene Therapy Vector: Cryo-Electron Microscopy at 4.5A resolution==
<StructureSection load='3j1q' size='340' side='right' caption='[[3j1q]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
<SX load='3j1q' size='340' side='right' viewer='molstar' caption='[[3j1q]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3j1q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Adeno-associated_virus Adeno-associated virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J1Q FirstGlance]. <br>
<table><tr><td colspan='2'>[[3j1q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Adeno-associated_virus Adeno-associated virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J1Q FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Capsid ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272636 Adeno-associated virus])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1q OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j1q RCSB], [http://www.ebi.ac.uk/pdbsum/3j1q PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1q OCA], [https://pdbe.org/3j1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j1q RCSB], [https://www.ebi.ac.uk/pdbsum/3j1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j1q ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q6JC41_9VIRU Q6JC41_9VIRU]
AAV-DJ, a leading candidate vector for liver gene therapy, was created through random homologous recombination followed by directed evolution, selecting for in vivo liver tropism and resistance to in vitro immune neutralization. Here, the 4.5 A resolution cryo-EM structure is determined for the engineered AAV vector, revealing structural features that illuminate its phenotype. The heparan sulfate receptor-binding site is little changed from AAV-2, and heparin-binding affinity is similar. A loop that is antigenic in other serotypes has a unique conformation in AAV-DJ that would conflict with the binding of an AAV-2 neutralizing monoclonal antibody. This is consistent with increased resistance to neutralization by human polyclonal sera, raising the possibility that changed tropism may be a secondary effect of altered immune interactions. The reconstruction exemplifies analysis of fine structural changes and the potential of cryo-EM, in favorable cases, to characterize mutant or ligand-bound complexes.
 
Structure of AAV-DJ, a Retargeted Gene Therapy Vector: Cryo-Electron Microscopy at 4.5 A Resolution.,Lerch TF, O'Donnell JK, Meyer NL, Xie Q, Taylor KA, Stagg SM, Chapman MS Structure. 2012 Aug 8;20(8):1310-20. Epub 2012 Jun 21. PMID:22727812<ref>PMID:22727812</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</SX>
[[Category: Adeno-associated virus]]
[[Category: Adeno-associated virus]]
[[Category: Chapman, M S]]
[[Category: Large Structures]]
[[Category: Donnell, J K.O]]
[[Category: Chapman MS]]
[[Category: Lerch, T F]]
[[Category: Lerch TF]]
[[Category: Meyer, N L]]
[[Category: Meyer NL]]
[[Category: Stagg, S M]]
[[Category: O'Donnell JK]]
[[Category: Taylor, K A]]
[[Category: Stagg SM]]
[[Category: Xie, Q]]
[[Category: Taylor KA]]
[[Category: Gene therapy]]
[[Category: Xie Q]]
[[Category: Virus]]

Latest revision as of 10:09, 21 February 2024

Structure of AAV-DJ, a Retargeted Gene Therapy Vector: Cryo-Electron Microscopy at 4.5A resolution

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