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==The crystal structure of Anabaena CcbP==
==The crystal structure of Anabaena CcbP==
<StructureSection load='3urg' size='340' side='right' caption='[[3urg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3urg' size='340' side='right'caption='[[3urg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3urg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_sp._pcc_7120 Nostoc sp. pcc 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3URG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3URG FirstGlance]. <br>
<table><tr><td colspan='2'>[[3urg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3URG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3URG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MNB:5-MERCAPTO-2-NITRO-BENZOIC+ACID'>MNB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.003&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2k2v|2k2v]], [[2p0p|2p0p]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MNB:5-MERCAPTO-2-NITRO-BENZOIC+ACID'>MNB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ccbP, alr1010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Nostoc sp. PCC 7120])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3urg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3urg OCA], [https://pdbe.org/3urg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3urg RCSB], [https://www.ebi.ac.uk/pdbsum/3urg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3urg ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3urg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3urg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3urg RCSB], [http://www.ebi.ac.uk/pdbsum/3urg PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8YY42_NOSS1 Q8YY42_NOSS1]
Obtaining crystals presented a bottleneck in the structural study of Anabaena cyanobacterial Ca(2+)-binding protein (CcbP). In this report, the promoting effect of Ellman's reagent [5,5'-dithiobis(2-nitrobenzoic acid); DTNB] on the crystallization of CcbP is described. CcbP contains one free cysteine. A quick and simple oxidation reaction with DTNB blocked the free cysteine in purified CcbP and generated a homogenous monomeric protein for crystallization. The crystal structure of DTNB-modified CcbP was determined by the single-wavelength anomalous diffraction method. Structure analysis indicated that DTNB modification facilitated crystallization of CcbP by inducing polar interactions in the crystal lattice. DTNB-mediated cysteine modification was demonstrated to have little effect on the overall structure and the Ca(2+) binding of CcbP. Thus, DTNB modification may provide a simple and general approach for protein modification to improve the success of crystallization screening.
 
Ellman's reagent in promoting crystallization and structure determination of Anabaena CcbP.,Fan XX, Zhou YF, Liu X, Li LF, Su XD Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1409-14., doi: 10.1107/S1744309112034938. Epub 2012 Oct 30. PMID:23143261<ref>PMID:23143261</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Nostoc sp. pcc 7120]]
[[Category: Large Structures]]
[[Category: Fan, X X]]
[[Category: Nostoc sp. PCC 7120 = FACHB-418]]
[[Category: Liu, X]]
[[Category: Fan XX]]
[[Category: Su, X D]]
[[Category: Liu X]]
[[Category: Calcium binding protein]]
[[Category: Su XD]]
[[Category: Metal binding protein]]
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