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==Hexameric ring of Methanococcus voltae RadA==
==Hexameric ring of Methanococcus voltae RadA==
<StructureSection load='4dc9' size='340' side='right' caption='[[4dc9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='4dc9' size='340' side='right'caption='[[4dc9]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4dc9]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DC9 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4dc9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanococcus_voltae Methanococcus voltae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DC9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DC9 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1t4g|1t4g]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">radA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2188 Methanococcus voltae])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc9 OCA], [https://pdbe.org/4dc9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dc9 RCSB], [https://www.ebi.ac.uk/pdbsum/4dc9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dc9 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dc9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dc9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dc9 RCSB], [http://www.ebi.ac.uk/pdbsum/4dc9 PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/RADA_METVO RADA_METVO] Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules (By similarity).
Archaeal RadA proteins are close homologues of eukaryal Rad51 and DMC1 proteins and are remote homologues of bacterial RecA proteins. For the repair of double-stranded breaks in DNA, these recombinases promote a pivotal strand-exchange reaction between homologous single-stranded and double-stranded DNA substrates. This DNA-repair function also plays a key role in the resistance of cancer cells to chemotherapy and radiotherapy and in the resistance of bacterial cells to antibiotics. A hexameric form of a truncated Methanococcus voltae RadA protein devoid of its small N-terminal domain has been crystallized. The RadA hexamers further assemble into two-ringed assemblies. Similar assemblies can be observed in the crystals of Pyrococcus furiosus RadA and Homo sapiens DMC1. In all of these two-ringed assemblies the DNA-interacting L1 region of each protomer points inward towards the centre, creating a highly positively charged locus. The electrostatic characteristics of the central channels can be utilized in the design of novel recombinase inhibitors.
 
Structure of a hexameric form of RadA recombinase from Methanococcus voltae.,Du L, Luo Y Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 May 1;68(Pt 5):511-6. doi:, 10.1107/S1744309112010226. Epub 2012 Apr 20. PMID:22691778<ref>PMID:22691778</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Resolvase 3D structures|Resolvase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Methanococcus voltae]]
[[Category: Methanococcus voltae]]
[[Category: Du, L]]
[[Category: Du L]]
[[Category: Luo, Y]]
[[Category: Luo Y]]
[[Category: Dna binding protein]]
[[Category: Hexamer]]
[[Category: Homologous recombination]]
[[Category: Rada]]
[[Category: Reca]]
[[Category: Recombinase]]

Latest revision as of 14:31, 14 March 2024

Hexameric ring of Methanococcus voltae RadA

4dc9, resolution 2.60Å

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