1o9z: Difference between revisions

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[[Image:1o9z.jpg|left|200px]]


{{Structure
==F17-aG lectin domain from Escherichia coli (ligand free)==
|PDB= 1o9z |SIZE=350|CAPTION= <scene name='initialview01'>1o9z</scene>, resolution 1.75&Aring;
<StructureSection load='1o9z' size='340' side='right'caption='[[1o9z]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1o9z]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O9Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O9Z FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o9z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o9z OCA], [https://pdbe.org/1o9z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o9z RCSB], [https://www.ebi.ac.uk/pdbsum/1o9z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o9z ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/F17AG_ECOLX F17AG_ECOLX] Essential fimbrial adhesion factor that mediates binding to N-acetylglucosamine-containing receptors in the host intestinal microvilli, leading to colonization of the intestinal tissue, and diarrhea or septicemia. Also confers adhesiveness to laminin and basement membranes.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The F17-G adhesin at the tip of flexible F17 fimbriae of enterotoxigenic Escherichia coli mediates binding to N-acetyl-beta-D-glucosamine-presenting receptors on the microvilli of the intestinal epithelium of ruminants. We report the 1.7 A resolution crystal structure of the lectin domain of F17-G, both free and in complex with N-acetylglucosamine. The monosaccharide is bound on the side of the ellipsoid-shaped protein in a conserved site around which all natural variations of F17-G are clustered. A model is proposed for the interaction between F17-fimbriated E. coli and microvilli with enhanced affinity compared with the binding constant we determined for F17-G binding to N-acetylglucosamine (0.85 mM-1). Unexpectedly, the F17-G structure reveals that the lectin domains of the F17-G, PapGII and FimH fimbrial adhesins all share the immunoglobulin-like fold of the structural components (pilins) of their fimbriae, despite lack of any sequence identity. Fold comparisons with pilin and chaperone structures of the chaperone/usher pathway highlight the central role of the C-terminal beta-strand G of the immunoglobulin-like fold and provides new insights into pilus assembly, function and adhesion.


'''F17-AG LECTIN DOMAIN FROM ESCHERICHIA COLI (LIGAND FREE)'''
The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine.,Buts L, Bouckaert J, De Genst E, Loris R, Oscarson S, Lahmann M, Messens J, Brosens E, Wyns L, De Greve H Mol Microbiol. 2003 Aug;49(3):705-15. PMID:12864853<ref>PMID:12864853</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1o9z" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The F17-G adhesin at the tip of flexible F17 fimbriae of enterotoxigenic Escherichia coli mediates binding to N-acetyl-beta-D-glucosamine-presenting receptors on the microvilli of the intestinal epithelium of ruminants. We report the 1.7 A resolution crystal structure of the lectin domain of F17-G, both free and in complex with N-acetylglucosamine. The monosaccharide is bound on the side of the ellipsoid-shaped protein in a conserved site around which all natural variations of F17-G are clustered. A model is proposed for the interaction between F17-fimbriated E. coli and microvilli with enhanced affinity compared with the binding constant we determined for F17-G binding to N-acetylglucosamine (0.85 mM-1). Unexpectedly, the F17-G structure reveals that the lectin domains of the F17-G, PapGII and FimH fimbrial adhesins all share the immunoglobulin-like fold of the structural components (pilins) of their fimbriae, despite lack of any sequence identity. Fold comparisons with pilin and chaperone structures of the chaperone/usher pathway highlight the central role of the C-terminal beta-strand G of the immunoglobulin-like fold and provides new insights into pilus assembly, function and adhesion.
*[[Adhesin 3D structures|Adhesin 3D structures]]
 
== References ==
==About this Structure==
<references/>
1O9Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O9Z OCA].
__TOC__
 
</StructureSection>
==Reference==
The fimbrial adhesin F17-G of enterotoxigenic Escherichia coli has an immunoglobulin-like lectin domain that binds N-acetylglucosamine., Buts L, Bouckaert J, De Genst E, Loris R, Oscarson S, Lahmann M, Messens J, Brosens E, Wyns L, De Greve H, Mol Microbiol. 2003 Aug;49(3):705-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12864853 12864853]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Bouckaert, J.]]
[[Category: Bouckaert J]]
[[Category: Brosens, E.]]
[[Category: Brosens E]]
[[Category: Buts, L.]]
[[Category: Buts L]]
[[Category: Genst, E De.]]
[[Category: De Genst E]]
[[Category: Greve, H De.]]
[[Category: De Greve H]]
[[Category: Lahmann, M.]]
[[Category: Lahmann M]]
[[Category: Loris, R.]]
[[Category: Loris R]]
[[Category: Messens, J.]]
[[Category: Messens J]]
[[Category: Oscarson, S.]]
[[Category: Oscarson S]]
[[Category: Wyns, L.]]
[[Category: Wyns L]]
[[Category: bacterial adhesin]]
[[Category: bacterial attachment]]
[[Category: immunoglobulin fold]]
[[Category: lectin]]
[[Category: pathogenesis]]
 
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Retrieved from "https://proteopedia.org/1o9z"