TolB: Difference between revisions

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~~{{STRUCTURE_1c5k| PDB~~=~~1c5k | SIZE~~=~~400| SCENE~~=TolB/Tolb/1 ~~|right|CAPTION~~=E. coli TolB, [[1c5k]] }}

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==The TolB-Pal Complex==

<structure load='2w8b' size='300' align='left' caption='Interaction of TolB (grey, green, yellow, cyan) and peptoglycan-associated lipoprotein (Pal) (pink, magenta, gold, red), sulfate, acetate and glycerol [[2w8b]]' (PDB entry [[2w8b]] >The TolB-[[Pal]] complex is involved in maintaining the outer membrane integrity. Upon binding, TolB and Pal undergo a conformational change , the result of which is crucial for further interactions with other proteins<ref name='Godlewska'>PMID: 19519769</ref>. This complex is parasitised by protein antibiotics and disrupted in order to trigger the translocation of the toxin across the outer membrane (see [[Colicin]] for further information)<ref name='Bonsor'>PMID: 17375930</ref>.

To study the interaction of TolB with Pal, two studies were carried out<ref name='Bouveret'>PMID 7744736</ref>: TolBBep (tagging TolB for immunoprecipitation), which allows the associated proteins to remain in contact with TolB, and ''in vivo'' cross-linking experiments with formaldehyde. Immunoprecipitation gave the result that Pal co-precipitates with TolBBep, while the cross-linking showed that in the present of Pal, the two products migrated close to each other, but in the absence of Pal, neither band was present, demonstrating an interaction between the two. These two experiments showed that TolB directly interacts with Pal, and that this interaction is responsible for maintaining the association of TolB with the membrane.

The TolB-[[Pal]] complex is involved in maintaining the outer membrane integrity. Upon binding, TolB and Pal undergo a conformational change , the result of which is crucial for further interactions with other proteins<ref name='Godlewska'>PMID: 19519769</ref>. This complex is parasitised by protein antibiotics and disrupted in order to trigger the translocation of the toxin across the outer membrane (see [[Colicin]] for further information)<ref name='Bonsor'>PMID: 17375930</ref>.

To study the interaction of TolB with Pal, two studies were carried out<ref name='Bouveret'>PMID 7744736</ref>: TolBBep (tagging TolB for immunoprecipitation), which allows the associated proteins to remain in contact with TolB, and ''in vivo'' cross-linking experiments with formaldehyde. Immunoprecipitation gave the result that Pal co-precipitates with TolBBep, while the cross-linking showed that in the present of Pal, the two products migrated close to each other, but in the absence of Pal, neither band was present, demonstrating an interaction between the two. These two experiments showed that TolB directly interacts with Pal, and that this interaction is responsible for maintaining the association of TolB with the membrane.

{{STRUCTURE_2hqs| PDB=2hqs | SIZE=300| SCENE= |left|CAPTION=E. coli TolB (cyan) complex with peptidoglycan-associated lipoprotein (gold), acetate, sulfate and glycerol, (PDB code = [[2hgs]]) }}

</StructureSection>

==3D structures of TolB==

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[[2ivz]] – EcTolB + colicin-E9 T-domain

[[4jml]] - EcTolB (mutant) + colicin-E9 T-domain (mutant)

[[7nst]] - EcTolB (mutant) + colicin-E9 + outer membrane protein F – Cryo EM

[[7nsu]] - EcTolB (mutant) + colicin-E9 + outer membrane protein F + BtuB – Cryo EM

[[3iax]] - EcTolB + colicin-A translocation domain

[[2hqs]], [[2w8b]] - EcTolB + peptidoglycan-associated lipoprotein

[[4pwz]] – ~~TolB~~ – ''Yersinia pestis''

[[4pwz]] – YpTolB – ''Yersinia pestis''

[[4r40]] - YpTolB + peptidoglycan-associated lipoprotein

[[6pnv]] – TolB – ''Salmonella enterica''

[[7mx5]] – TolB – ''Acinetobacteria baumannii''

== References==

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@@ Line 1: / Line 1: @@
-{{STRUCTURE_1c5k|  PDB=1c5k  | SIZE=400| SCENE=TolB/Tolb/1 |right|CAPTION=E. coli TolB, [[1c5k]] }}
+<StructureSection load='' size='350' side='right' scene='TolB/Tolb/1' caption='E. coli TolB (PDB code [[1c5k]])'>
@@ Line 11: / Line 11: @@
 ==The TolB-Pal Complex==
-<structure load='2w8b' size='300' align='left' caption='Interaction of TolB (grey, green, yellow, cyan) and peptoglycan-associated lipoprotein (Pal) (pink, magenta, gold, red), sulfate, acetate and glycerol [[2w8b]]' (PDB entry [[2w8b]] >The TolB-[[Pal]] complex is involved in maintaining the outer membrane integrity.  Upon binding, TolB and Pal undergo a conformational change , the result of which is crucial for further interactions with other proteins<ref name='Godlewska'>PMID: 19519769</ref>.  This complex is parasitised by protein antibiotics  and disrupted in order to trigger the translocation of the toxin across the outer membrane (see [[Colicin]] for further information)<ref name='Bonsor'>PMID: 17375930</ref>.
-To study the interaction of TolB with Pal, two studies were carried out<ref name='Bouveret'>PMID 7744736</ref>: TolBBep (tagging TolB for immunoprecipitation), which allows the associated proteins to remain in contact with TolB, and ''in vivo'' cross-linking experiments with formaldehyde.  Immunoprecipitation gave the result that Pal co-precipitates with TolBBep, while the cross-linking showed that in the present of Pal, the two products migrated close to each other, but in the absence of Pal, neither band was present, demonstrating an interaction between the two.  These two experiments showed that TolB directly interacts with Pal, and that this interaction is responsible for maintaining the association of TolB with the membrane.
+The TolB-[[Pal]] complex is involved in maintaining the outer membrane integrity.  Upon binding, TolB and Pal undergo a conformational change , the result of which is crucial for further interactions with other proteins<ref name='Godlewska'>PMID: 19519769</ref>.  This complex is parasitised by protein antibiotics  and disrupted in order to trigger the translocation of the toxin across the outer membrane (see [[Colicin]] for further information)<ref name='Bonsor'>PMID: 17375930</ref>.
+To study the interaction of TolB with Pal, two studies were carried out<ref name='Bouveret'>PMID 7744736</ref>: TolBBep (tagging TolB for immunoprecipitation), which allows the associated proteins to remain in contact with TolB, and ''in vivo'' cross-linking experiments with formaldehyde.  Immunoprecipitation gave the result that Pal co-precipitates with TolBBep, while the cross-linking showed that in the present of Pal, the two products migrated close to each other, but in the absence of Pal, neither band was present, demonstrating an interaction between the two.  These two experiments showed that TolB directly interacts with Pal, and that this interaction is responsible for maintaining the association of TolB with the membrane.
+{{STRUCTURE_2hqs|  PDB=2hqs  | SIZE=300| SCENE= |left|CAPTION=E. coli TolB (cyan) complex with peptidoglycan-associated lipoprotein (gold), acetate, sulfate and glycerol, (PDB code = [[2hgs]]) }}
+</StructureSection>
 ==3D structures of TolB==
@@ Line 30: / Line 26: @@
 [[2ivz]] – EcTolB + colicin-E9 T-domain<br />
 [[4jml]] - EcTolB (mutant) + colicin-E9 T-domain (mutant)<br />
+[[7nst]] - EcTolB (mutant) + colicin-E9 + outer membrane protein F – Cryo EM<br />
+[[7nsu]] - EcTolB (mutant) + colicin-E9 + outer membrane protein F + BtuB – Cryo EM<br />
 [[3iax]] - EcTolB + colicin-A translocation domain<br />
 [[2hqs]], [[2w8b]] - EcTolB + peptidoglycan-associated lipoprotein<br />
-[[4pwz]] – TolB – ''Yersinia pestis''<br />
+[[4pwz]] – YpTolB – ''Yersinia pestis''<br />
+[[4r40]] - YpTolB + peptidoglycan-associated lipoprotein<br />
+[[6pnv]] – TolB – ''Salmonella enterica''<br />
+[[7mx5]] – TolB – ''Acinetobacteria baumannii''<br />
 == References==