Tryptophan synthase: Difference between revisions

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~~{{STRUCTURE_1wbj| PDB~~=~~1wbj | SIZE~~=~~400| SCENE~~=|right~~|CAPTION~~=Tryptophan synthase α subunit (~~grey~~) and β subunit (green) of the α2β2 tetramer complex with pyridoxal-phosphate, glycerol-3-phosphate and Na+ ion (purple) [[1wbj]]}}

'''Tryptophan synthase''' (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan. TrpS α subunit catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP). The β subunit catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine. The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits. ~~TrpS is not found in animals hence it is tested as a possible drug target for tubertculosis~~, ~~ocular and genital infections, cryptosporidiosis and as herbicide~~.

== Function ==

'''Tryptophan synthase''' (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan (Trp)<ref>PMID:11893063</ref>. TrpS '''α subunit''' catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP). The '''β subunit''' catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine. The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits. '''Dimethylallyl tryptophan synthase''' (DMTS) catalyzes the alkylation of Trp by dimethylallyl phosphate to form 4-(γ,γ-dimethylallyl)-Trp<ref>PMID:1605639</ref>.

== Relevance ==

TrpS is not found in animals hence it is tested as a possible drug target for tuberculosis, ocular and genital infections, cryptosporidiosis and as herbicide<ref>PMID:19430702</ref>.

== Structural highlights ==

The <scene name='52/525189/Cv/6'>active site</scene> of TrpS subunit α contains the catalytic residues Glu and Asp<ref>PMID:16120446</ref>. Water molecules are shown as red spheres.

==3D structures of tryptophan synthase==

[[Tryptophan synthase 3D structures]]

~~Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}~~

</StructureSection>

~~{{#tree:id=OrganizedByTopic|openlevels=0|~~

*'''Tryptophan synthase α subunit'''

**[[3vnd]] - TrpS α subunit – ''Shewanella frigidimarina''

**[[3tha]] - TrpS α subunit – ''Campylobacter jejuni''

**[[3nav]] - TrpS α subunit – ''Vibrio cholerae''

**[[1geq]] - PfTrpS α subunit - ''Pyrococcus furiosus''

**[[2dzp]], [[2dzs]], [[2dzt]], [[2dzu]], [[2dzv]], [[2dzw]], [[2dzx]], [[2e09]] - PfTrpS α subunit (mutant)

**[[1rd5]] - TrpS α subunit – corn

**[[1ujp]] - TtTrpS α subunit

**[[1v7y]], [[1wq5]], [[1xc4]] - EcTrpS α subunit

**[[1xcf]] - EcTrpS α subunit (mutant)

**[[2ekc]] - TrpS α subunit – ''Aquifex aeolicus''

*Tryptophan synthase α binary complex

**[[1wxj]] - TtTrpS α subunit + indole propanol phosphate

*'''Tryptophan synthase β subunit'''

**[[1x1q]] - TtTrpS β subunit – ''Thermus thermophilus''

**[[2dh6]] - EcTrpS β subunit]] - ''Escherichia coli''

**[[2o2e]], [[2o2j]] - TrpS β subunit – ''Mycobacterium tuberculosis''

**[[1v8z]] - PfTrpS β subunit

**[[4neg]] - TrpS β subunit – ''Bacillus anthracis''

*Tryptophan synthase β binary complex

**[[2dh5]] - EcTrpS β subunit + PLP

*'''Tryptophan synthase α+β subunits'''

**[[1wdw]] - PfTrpS α + β subunits

*Tryptophan synthase α+β binary complex

**[[1ttp]], [[1ttq]], [[1bks]], [[2wsy]], [[1kfk]] - StTrpS α + β + PLP – ''Salmonella typhimurium''

**[[1a5a]], [[1k8x]] - StTrpS α (mutant) + β + PLP

**[[1k7x]], [[2j9z]] - StTrpS α + β (mutant) + PLP

**[[2tys]] - StTrpS α (mutant) + β (mutant) + Trp derivative

**[[1kfe]] - StTrpS α (mutant) + β + pyridoxal-serine-monophsphate

**[[1kfj]] - StTrpS α + β + pyridoxal-serine-monophsphate

**[[2j9y]] - StTrpS α + β (mutant) + enoic acid derivative

**[[3pr2]] - StTrpS α + β + inhibitor

**[[4hn4]], [[4hpj]], [[4hpx]], [[4ht3]], [[4kkx]] - TrpS α + β + inhibitor – ''Salmonella enterica''

*Tryptophan synthase α+β ternary complex with PLP and inhibitor

**[[1a50]], [[1cw2]], [[1c9d]], [[1cx9]], [[1c8v]], [[1c29]], [[2cle]], [[2clh]], [[2cli]], [[2clk]], [[2cll]], [[2clm]], [[2clo]], [[2clf]] - StTrpS α + β + inhibitor + PLP

**[[1fuy]] - StTrpS α + β (mutant) + inhibitor + PLP

*Tryptophan synthase α+β ternary complex with PLP and IGP derivatives

**[[1a5b]], [[1kfb]] - StTrpS α (mutant) + β + IGP + PLP

**[[1k8z]] - StTrpS α + β (mutant) + IGP derivative + PLP

**[[1kfc]] - StTrpS α (mutant) + β + IGP derivative + PLP

**[[1k3u]], [[1k7e]], [[1k7f]] - StTrpS α + β + IGP derivative + PLP

**[[1qoq]], [[2rh9]], [[2rhg]] - StTrpS α + β + IGP + PLP

**[[1qop]] - StTrpS α + β + IGP analog + PLP

**[[2trs]], [[1beu]] - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + IGP analog

*Tryptophan synthase α+β ternary complex with PLP and G3P derivatives

**[[1wbj]] - StTrpS α + β + G3P + PLP

**[[2j9x]] - StTrpS α + β + G3P + acrylic acid derivative

**[[1k8y]] - StTrpS α + β (mutant) + G3P derivative + PLP

**[[1tjp]] - StTrpS α + β + G3P derivative + PLP

**[[2tsy]] - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + G3P

**[[1ubs]] - StTrpS α (mutant) + β (mutant) + serine + PLP

*Tryptophan synthase α+β quaternary complex

**[[1a5s]] - StTrpS α + β + serine + inhibitor + PLP

== References ==

**[[3cep]] - StTrpS α + β + G3P + indoline + PLP

}}

[[Category:Topic Page]]

@@ Line 1: / Line 1: @@
-{{STRUCTURE_1wbj|  PDB=1wbj  | SIZE=400| SCENE=|right|CAPTION=Tryptophan synthase α subunit (grey) and β subunit (green) of the α2β2 tetramer complex with pyridoxal-phosphate, glycerol-3-phosphate and Na+ ion (purple) [[1wbj]]}}
+<StructureSection load='' size='350' side='right' caption='Tryptophan synthase α subunit (yellow, salmon) and β subunit (cyan, green) of the α2β2 tetramer complex with pyridoxal-phosphate, glycerol-3-phosphate and Na+ ion (purple) [[1wbj]]' scene='52/525189/Cv/5'>
-'''Tryptophan synthase''' (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan.  TrpS α subunit catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP).  The β subunit catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine.  The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits.  TrpS is not found in animals hence it is tested as a possible drug target for tubertculosis, ocular and genital infections, cryptosporidiosis and as herbicide.
+== Function ==
+'''Tryptophan synthase''' (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan (Trp)<ref>PMID:11893063</ref>.  TrpS '''α subunit''' catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP).  The '''β subunit''' catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine.  The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits.  '''Dimethylallyl tryptophan synthase''' (DMTS) catalyzes the alkylation of Trp by dimethylallyl phosphate to form 4-(γ,γ-dimethylallyl)-Trp<ref>PMID:1605639</ref>.
+== Relevance ==
+TrpS is not found in animals hence it is tested as a possible drug target for tuberculosis, ocular and genital infections, cryptosporidiosis and as herbicide<ref>PMID:19430702</ref>.
+== Structural highlights ==
+The <scene name='52/525189/Cv/6'>active site</scene> of TrpS subunit α contains the catalytic residues Glu and Asp<ref>PMID:16120446</ref>. Water molecules are shown as red spheres.
 ==3D structures of tryptophan synthase==
+[[Tryptophan synthase 3D structures]]
-Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+</StructureSection>
-{{#tree:id=OrganizedByTopic|openlevels=0|
-*'''Tryptophan synthase α subunit'''
-**[[3vnd]] - TrpS α subunit – ''Shewanella frigidimarina''<br />
-**[[3tha]] - TrpS α subunit – ''Campylobacter jejuni''<br />
-**[[3nav]] - TrpS α subunit – ''Vibrio cholerae''<br />
-**[[1geq]] - PfTrpS α subunit - ''Pyrococcus furiosus''<br />
-**[[2dzp]], [[2dzs]], [[2dzt]], [[2dzu]], [[2dzv]], [[2dzw]], [[2dzx]], [[2e09]] - PfTrpS α subunit (mutant)<br />
-**[[1rd5]] - TrpS α subunit – corn<br />
-**[[1ujp]] - TtTrpS α subunit <br />
-**[[1v7y]], [[1wq5]], [[1xc4]] - EcTrpS α subunit <br />
-**[[1xcf]] - EcTrpS α subunit (mutant)<br />
-**[[2ekc]] - TrpS α subunit – ''Aquifex aeolicus''<br />
-*Tryptophan synthase α binary complex
-**[[1wxj]] - TtTrpS α subunit + indole propanol phosphate<br />
-*'''Tryptophan synthase β subunit'''
-**[[1x1q]] - TtTrpS β subunit – ''Thermus thermophilus''<br />
-**[[2dh6]] - EcTrpS β subunit]] - ''Escherichia coli''<br />
-**[[2o2e]], [[2o2j]] - TrpS β subunit – ''Mycobacterium tuberculosis''<br />
-**[[1v8z]] - PfTrpS β subunit <br />
-**[[4neg]] - TrpS β subunit – ''Bacillus anthracis''<br />
-*Tryptophan synthase β binary complex
-**[[2dh5]] - EcTrpS β subunit + PLP<br />
-*'''Tryptophan synthase α+β subunits'''
-**[[1wdw]] - PfTrpS α + β subunits <br />
-*Tryptophan synthase α+β binary complex
-**[[1ttp]], [[1ttq]], [[1bks]], [[2wsy]], [[1kfk]] - StTrpS α + β + PLP – ''Salmonella typhimurium''<br />
-**[[1a5a]], [[1k8x]] - StTrpS α (mutant) + β + PLP<br />
-**[[1k7x]], [[2j9z]] - StTrpS α + β (mutant) + PLP<br />
-**[[2tys]] - StTrpS α (mutant) + β (mutant) + Trp derivative<br />
-**[[1kfe]] - StTrpS α (mutant) + β + pyridoxal-serine-monophsphate<br />
-**[[1kfj]] - StTrpS α + β + pyridoxal-serine-monophsphate<br />
-**[[2j9y]] - StTrpS α + β (mutant) + enoic acid derivative<br />
-**[[3pr2]] - StTrpS α + β + inhibitor<br />
-**[[4hn4]], [[4hpj]], [[4hpx]], [[4ht3]], [[4kkx]] - TrpS α + β + inhibitor – ''Salmonella enterica''<br />
-*Tryptophan synthase α+β ternary complex with PLP and inhibitor
-**[[1a50]], [[1cw2]], [[1c9d]], [[1cx9]], [[1c8v]], [[1c29]], [[2cle]], [[2clh]], [[2cli]], [[2clk]], [[2cll]], [[2clm]], [[2clo]], [[2clf]] - StTrpS α + β + inhibitor + PLP<br />
-**[[1fuy]] - StTrpS α + β (mutant) + inhibitor + PLP<br />
-*Tryptophan synthase α+β ternary complex with PLP and IGP derivatives
-**[[1a5b]], [[1kfb]] - StTrpS α (mutant) + β + IGP + PLP<br />
-**[[1k8z]] - StTrpS α + β (mutant) + IGP derivative + PLP<br />
-**[[1kfc]] - StTrpS α (mutant) + β + IGP derivative + PLP<br />
-**[[1k3u]], [[1k7e]], [[1k7f]] - StTrpS α + β + IGP derivative + PLP<br />
-**[[1qoq]], [[2rh9]], [[2rhg]] - StTrpS α + β + IGP + PLP<br />
-**[[1qop]] - StTrpS α + β + IGP analog + PLP<br />
-**[[2trs]], [[1beu]] - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + IGP analog
-*Tryptophan synthase α+β ternary complex with PLP and G3P derivatives
-**[[1wbj]] - StTrpS α + β + G3P + PLP<br />
-**[[2j9x]] - StTrpS α + β + G3P + acrylic acid derivative<br />
-**[[1k8y]] - StTrpS α + β (mutant) + G3P derivative + PLP<br />
-**[[1tjp]] - StTrpS α + β + G3P derivative + PLP<br />
-**[[2tsy]] - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + G3P
-**[[1ubs]] - StTrpS α (mutant) + β (mutant) + serine + PLP<br />
-*Tryptophan synthase α+β quaternary complex
-**[[1a5s]] - StTrpS α + β + serine + inhibitor + PLP<br />
+== References ==
-**[[3cep]] - StTrpS α + β + G3P + indoline + PLP<br />
+<references/>
-}}
 [[Category:Topic Page]]

Tryptophan synthase: Difference between revisions

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