4rx6: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 4rx6 is ~~ON HOLD~~

==Structure of B. subtilis GlnK-ATP complex to 2.6 Angstrom==

== Structural highlights ==

<table><tr><td colspan='2'>[[4rx6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RX6 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5994Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rx6 OCA], [https://pdbe.org/4rx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rx6 RCSB], [https://www.ebi.ac.uk/pdbsum/4rx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rx6 ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/NRGB_BACSU NRGB_BACSU] Required for full induction of the nrgAB operon under conditions of ammonium limitation.<ref>PMID:14600241</ref>

== Publication Abstract from PubMed ==

All cells must sense and adapt to changing nutrient availability. However, detailed molecular mechanisms coordinating such regulatory pathways remain poorly understood. In Bacillus subtilis, nitrogen homeostasis is controlled by a unique circuitry composed of the regulator TnrA, which is deactivated by feedback-inhibited glutamine synthetase (GS) during nitrogen excess and stabilized by GlnK upon nitrogen depletion, and the repressor GlnR. Here we describe a complete molecular dissection of this network. TnrA and GlnR, the global nitrogen homeostatic transcription regulators, are revealed as founders of a new structural family of dimeric DNA-binding proteins with C-terminal, flexible, effector-binding sensors that modulate their dimerization. Remarkably, the TnrA sensor domains insert into GS intersubunit catalytic pores, destabilizing the TnrA dimer and causing an unprecedented GS dodecamer-to-tetradecamer conversion, which concomitantly deactivates GS. In contrast, each subunit of the GlnK trimer "templates" active TnrA dimers. Unlike TnrA, GlnR sensors mediate an autoinhibitory dimer-destabilizing interaction alleviated by GS, which acts as a GlnR chaperone. Thus, these studies unveil heretofore unseen mechanisms by which inducible sensor domains drive metabolic reprograming in the model Gram-positive bacterium B. subtilis.

~~Authors:~~ Schumacher, ~~M.A.~~, Cuthbert, B., Tonthat, N.~~, Chinnam, N~~.G.~~, Whitfill, T~~.

Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis.,Schumacher MA, Chinnam NB, Cuthbert B, Tonthat NK, Whitfill T Genes Dev. 2015 Feb 15;29(4):451-64. doi: 10.1101/gad.254714.114. PMID:25691471<ref>PMID:25691471</ref>

~~Description~~: ~~Structure of B~~. subtilis ~~GlnK-ATP complex to 2~~.~~6 Angstrom~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Bacillus subtilis subsp. subtilis str. 168]]

[[Category: Large Structures]]

[[Category: Chinnam NG]]

[[Category: Cuthbert B]]

[[Category: Schumacher MA]]

[[Category: Tonthat N]]

[[Category: Whitfill T]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4rx6 is ON HOLD
+==Structure of B. subtilis GlnK-ATP complex to 2.6 Angstrom==
+<StructureSection load='4rx6' size='340' side='right'caption='[[4rx6]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4rx6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RX6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5994&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rx6 OCA], [https://pdbe.org/4rx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rx6 RCSB], [https://www.ebi.ac.uk/pdbsum/4rx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rx6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NRGB_BACSU NRGB_BACSU] Required for full induction of the nrgAB operon under conditions of ammonium limitation.<ref>PMID:14600241</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+All cells must sense and adapt to changing nutrient availability. However, detailed molecular mechanisms coordinating such regulatory pathways remain poorly understood. In Bacillus subtilis, nitrogen homeostasis is controlled by a unique circuitry composed of the regulator TnrA, which is deactivated by feedback-inhibited glutamine synthetase (GS) during nitrogen excess and stabilized by GlnK upon nitrogen depletion, and the repressor GlnR. Here we describe a complete molecular dissection of this network. TnrA and GlnR, the global nitrogen homeostatic transcription regulators, are revealed as founders of a new structural family of dimeric DNA-binding proteins with C-terminal, flexible, effector-binding sensors that modulate their dimerization. Remarkably, the TnrA sensor domains insert into GS intersubunit catalytic pores, destabilizing the TnrA dimer and causing an unprecedented GS dodecamer-to-tetradecamer conversion, which concomitantly deactivates GS. In contrast, each subunit of the GlnK trimer "templates" active TnrA dimers. Unlike TnrA, GlnR sensors mediate an autoinhibitory dimer-destabilizing interaction alleviated by GS, which acts as a GlnR chaperone. Thus, these studies unveil heretofore unseen mechanisms by which inducible sensor domains drive metabolic reprograming in the model Gram-positive bacterium B. subtilis.
-Authors: Schumacher, M.A., Cuthbert, B., Tonthat, N., Chinnam, N.G., Whitfill, T.
+Structures of regulatory machinery reveal novel molecular mechanisms controlling B. subtilis nitrogen homeostasis.,Schumacher MA, Chinnam NB, Cuthbert B, Tonthat NK, Whitfill T Genes Dev. 2015 Feb 15;29(4):451-64. doi: 10.1101/gad.254714.114. PMID:25691471<ref>PMID:25691471</ref>
-Description: Structure of B. subtilis GlnK-ATP complex to 2.6 Angstrom
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4rx6" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Bacillus subtilis subsp. subtilis str. 168]]
+[[Category: Large Structures]]
+[[Category: Chinnam NG]]
+[[Category: Cuthbert B]]
+[[Category: Schumacher MA]]
+[[Category: Tonthat N]]
+[[Category: Whitfill T]]