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[[Image:1pjf.jpg|left|200px]]


{{Structure
==Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage==
|PDB= 1pjf |SIZE=350|CAPTION= <scene name='initialview01'>1pjf</scene>
<StructureSection load='1pjf' size='340' side='right'caption='[[1pjf]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1pjf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_virus_Pf1 Pseudomonas virus Pf1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJF FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjf OCA], [https://pdbe.org/1pjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjf RCSB], [https://www.ebi.ac.uk/pdbsum/1pjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjf ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/CAPSD_BPPF1 CAPSD_BPPF1] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity).


'''Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage'''
==See Also==
 
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
The atomic resolution structure of Pf1 coat protein determined by solid-state NMR spectroscopy of magnetically aligned filamentous bacteriophage particles in solution is compared to the structures previously determined by X-ray fiber and neutron diffraction, the structure of its membrane-bound form, and the structure of fd coat protein. These structural comparisons provide insights into several biological properties, differences between class I and class II filamentous bacteriophages, and the assembly process. The six N-terminal amino acid residues adopt an unusual "double hook" conformation on the outside of the bacteriophage particle. The solid-state NMR results indicate that at 30 degrees C, some of the coat protein subunits assume a single, fully structured conformation, and some have a few mobile residues that provide a break between two helical segments, in agreement with structural models from X-ray fiber and neutron diffraction, respectively. The atomic resolution structure determined by solid-state NMR for residues 7-14 and 18-46, which excludes the N-terminal double hook and the break between the helical segments, but encompasses more than 80% of the backbone including the distinct kink at residue 29, agrees with that determined by X-ray fiber diffraction with an RMSD value of 2.0 A. The symmetry and distance constraints determined by X-ray fiber and neutron diffraction enable the construction of an accurate model of the bacteriophage particle from the coordinates of the coat protein monomers.
[[Category: Large Structures]]
 
[[Category: Pseudomonas virus Pf1]]
==About this Structure==
[[Category: Nevzorov AA]]
1PJF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_pf1 Pseudomonas phage pf1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJF OCA].
[[Category: Opella SJ]]
 
[[Category: Thiriot DS]]
==Reference==
[[Category: Wu CH]]
Structure of the coat protein in Pf1 bacteriophage determined by solid-state NMR spectroscopy., Thiriot DS, Nevzorov AA, Zagyanskiy L, Wu CH, Opella SJ, J Mol Biol. 2004 Aug 13;341(3):869-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15288792 15288792]
[[Category: Zagyanskiy L]]
[[Category: Pseudomonas phage pf1]]
[[Category: Single protein]]
[[Category: Nevzorov, A A.]]
[[Category: Opella, S J.]]
[[Category: Thiriot, D S.]]
[[Category: Wu, C H.]]
[[Category: Zagyanskiy, L.]]
[[Category: helical virus]]
[[Category: virus/viral protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:25:08 2008''

Latest revision as of 06:33, 1 May 2024

Solid State NMR structure of the Pf1 Major Coat Protein in Magnetically Aligned Bacteriophage

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