1pw4: Difference between revisions

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[[Image:1pw4.jpg|left|200px]]


{{Structure
==Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli==
|PDB= 1pw4 |SIZE=350|CAPTION= <scene name='initialview01'>1pw4</scene>, resolution 3.3&Aring;
<StructureSection load='1pw4' size='340' side='right'caption='[[1pw4]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1pw4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PW4 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
|GENE= glpT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pw4 OCA], [https://pdbe.org/1pw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pw4 RCSB], [https://www.ebi.ac.uk/pdbsum/1pw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pw4 ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal Structure of the Glycerol-3-Phosphate Transporter from E.Coli'''
[https://www.uniprot.org/uniprot/GLPT_ECOLI GLPT_ECOLI] Responsible for glycerol-3-phosphate uptake.
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
The major facilitator superfamily represents the largest group of secondary membrane transporters in the cell. Here we report the 3.3 angstrom resolution structure of a member of this superfamily, GlpT, which transports glycerol-3-phosphate into the cytoplasm and inorganic phosphate into the periplasm. The amino- and carboxyl-terminal halves of the protein exhibit a pseudo two-fold symmetry. Closed off to the periplasm, a centrally located substrate-translocation pore contains two arginines at its closed end, which comprise the substrate-binding site. Upon substrate binding, the protein adopts a more compact conformation. We propose that GlpT operates by a single-binding site, alternating-access mechanism through a rocker-switch type of movement.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pw/1pw4_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
1PW4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PW4 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pw4 ConSurf].
Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli., Huang Y, Lemieux MJ, Song J, Auer M, Wang DN, Science. 2003 Aug 1;301(5633):616-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12893936 12893936]
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Auer, M.]]
[[Category: Auer M]]
[[Category: Huang, Y.]]
[[Category: Huang Y]]
[[Category: Lemieux, M J.]]
[[Category: Lemieux MJ]]
[[Category: Song, J.]]
[[Category: Song J]]
[[Category: Wang, D N.]]
[[Category: Wang DN]]
[[Category: glycerol-3-phosphate]]
[[Category: inner membrane]]
[[Category: major facilitator superfamily]]
[[Category: secondary active membrane transporter]]
[[Category: transmembrane]]
[[Category: transporter]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:29:46 2008''
Retrieved from "https://proteopedia.org/1pw4"