4k0d: Difference between revisions
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==Periplasmic sensor domain of sensor histidine kinase, Adeh_2942== | ==Periplasmic sensor domain of sensor histidine kinase, Adeh_2942== | ||
<StructureSection load='4k0d' size='340' side='right' caption='[[4k0d]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='4k0d' size='340' side='right'caption='[[4k0d]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4k0d]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4k0d]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Anaeromyxobacter_dehalogenans_2CP-C Anaeromyxobacter dehalogenans 2CP-C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K0D FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand= | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.997Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k0d OCA], [https://pdbe.org/4k0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k0d RCSB], [https://www.ebi.ac.uk/pdbsum/4k0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k0d ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q2IDQ5_ANADE Q2IDQ5_ANADE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Anaeromyxobacter dehalogenans is a delta-proteobacterium found in diverse soils and sediments. It is of interest in bioremediation efforts due to its dechlorination and metal-reducing capabilities. To gain an understanding on A. dehalogenans' abilities to adapt to diverse environments we analyzed its signal transduction proteins. The A. dehalogenans genome codes for a large number of sensor histidine kinases (HK) and methyl-accepting chemotaxis proteins (MCP); among these 23 HK and 11 MCP proteins have a sensor domain in the periplasm. These proteins most likely contribute to adaptation to the organism's surroundings. We predicted their three-dimensional folds and determined the structures of two of the periplasmic sensor domains by X-ray diffraction. Most of the domains are predicted to have either PAS-like or helical bundle structures, with two predicted to have solute-binding protein fold, and another predicted to have a 6-phosphogluconolactonase like fold. Atomic structures of two sensor domains confirmed the respective fold predictions. The Adeh_2942 sensor (HK) was found to have a helical bundle structure, and the Adeh_3718 sensor (MCP) has a PAS-like structure. Interestingly, the Adeh_3718 sensor has an acetate moiety bound in a binding site typical for PAS-like domains. Future work is needed to determine whether Adeh_3718 is involved in acetate sensing by A. dehalogenans. | |||
Analysis of periplasmic sensor domains from Anaeromyxobacter dehalogenans 2CP-C: structure of one sensor domain from a histidine kinase and another from a chemotaxis protein.,Pokkuluri PR, Dwulit-Smith J, Duke NE, Wilton R, Mack JC, Bearden J, Rakowski E, Babnigg G, Szurmant H, Joachimiak A, Schiffer M Microbiologyopen. 2013 Oct;2(5):766-77. doi: 10.1002/mbo3.112. Epub 2013 Jul 30. PMID:23897711<ref>PMID:23897711</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4k0d" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Anaeromyxobacter dehalogenans | [[Category: Anaeromyxobacter dehalogenans 2CP-C]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Bearden J]] | ||
[[Category: | [[Category: Joachimiak A]] | ||
[[Category: Mack | [[Category: Mack JC]] | ||
[[Category: Pokkuluri | [[Category: Pokkuluri PR]] | ||
[[Category: Rakowski | [[Category: Rakowski E]] | ||
[[Category: Schiffer | [[Category: Schiffer M]] | ||
