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[[Image:1sjj.gif|left|200px]]


{{Structure
==Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin==
|PDB= 1sjj |SIZE=350|CAPTION= <scene name='initialview01'>1sjj</scene>
<StructureSection load='1sjj' size='340' side='right'caption='[[1sjj]], [[Resolution|resolution]] 20.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1sjj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SJJ FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 20&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sjj OCA], [https://pdbe.org/1sjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sjj RCSB], [https://www.ebi.ac.uk/pdbsum/1sjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sjj ProSAT]</span></td></tr>
}}
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACTN1_CHICK ACTN1_CHICK] F-actin cross-linking protein is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sj/1sjj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sjj ConSurf].
<div style="clear:both"></div>


'''Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin'''
==See Also==
 
*[[Actinin 3D structures|Actinin 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm resolution of 2-D arrays of smooth muscle alpha-actinin. The reconstruction reveals a well-resolved long central domain with 90 degrees of left-handed twist and near 2-fold symmetry. However, the molecular ends which contain the actin binding and calmodulin-like domains, have different structures oriented approximately 90 degrees to each other. Atomic structures for the alpha-actinin domains were built by homology modeling and assembled into an atomic model. Model building suggests that in the 2-D arrays, the two calponin homology domains that comprise the actin-binding domain have a closed conformation at one end and an open conformation at the other end due to domain swapping. The open and closed conformations of the actin-binding domain suggests flexibility that may underlie Ca2+ regulation. The approximately 90 degrees orientation difference at the molecular ends may underlie alpha-actinin's ability to crosslink actin filaments in nearly any orientation.
 
==About this Structure==
1SJJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJJ OCA].
 
==Reference==
A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region., Liu J, Taylor DW, Taylor KA, J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15050827 15050827]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Liu, J.]]
[[Category: Liu J]]
[[Category: Taylor, D W.]]
[[Category: Taylor DW]]
[[Category: Taylor, K A.]]
[[Category: Taylor KA]]
[[Category: 3-helix bundle]]
[[Category: actin binding protein]]
[[Category: calmodulin-like domain]]
[[Category: calponin homology domain]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:05:49 2008''
Retrieved from "https://proteopedia.org/1sjj"