4jkn: Difference between revisions
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==Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A== | ==Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A== | ||
<StructureSection load='4jkn' size='340' side='right' caption='[[4jkn]], [[Resolution|resolution]] 1.54Å' scene=''> | <StructureSection load='4jkn' size='340' side='right'caption='[[4jkn]], [[Resolution|resolution]] 1.54Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4jkn]] is a 4 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4jkn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JKN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JKN FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.536Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jkn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jkn OCA], [https://pdbe.org/4jkn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jkn RCSB], [https://www.ebi.ac.uk/pdbsum/4jkn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jkn ProSAT]</span></td></tr> | ||
< | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Mercury(II) metallation of Pseudomonas aeruginosa azurin has been characterized structurally and biochemically. The X-ray crystal structure at 1.5A of mercury(II) metallated azurin confirms the coordination of mercury at the copper binding active site and a second surface site. These findings are further validated by NMR, Matrix-assisted laser desorption/ionization spectrometry (MALDI), and UV-visible spectroscopic methods indicating copper displacement from the wild-type protein. Bioinformatic analysis has identified homologous human protein domains computationally, and compared them to the structure of azurin, providing a model for human mercury interactions. Study of the mercury-azurin adduct, in combination with other known examples of protein-heavy metal interactions, could provide further insight into the chemical mechanisms of toxicological interactions, leading toward a global understanding of the biological speciation of toxic heavy metals. | |||
Mercury metallation of the copper protein azurin and structural insight into possible heavy metal reactivity.,Zampino AP, Masters FM, Bladholm EL, Panzner MJ, Berry SM, Leeper TC, Ziegler CJ J Inorg Biochem. 2014 Dec;141:152-60. doi: 10.1016/j.jinorgbio.2014.09.003. Epub , 2014 Sep 16. PMID:25265377<ref>PMID:25265377</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4jkn" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Azurin|Azurin]] | *[[Azurin 3D structures|Azurin 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: | [[Category: Berry SB]] | ||
[[Category: | [[Category: Bladholm EL]] | ||
[[Category: | [[Category: Masters FM]] | ||
[[Category: | [[Category: Panzner MJ]] | ||
[[Category: | [[Category: Zampino AP]] | ||
[[Category: | [[Category: Ziegler CJ]] | ||
Latest revision as of 15:44, 20 September 2023
Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A
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