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[[Image:1tex.jpg|left|200px]]


{{Structure
==Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose==
|PDB= 1tex |SIZE=350|CAPTION= <scene name='initialview01'>1tex</scene>, resolution 2.60&Aring;
<StructureSection load='1tex' size='340' side='right'caption='[[1tex]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=TRE:TREHALOSE'>TRE</scene>
<table><tr><td colspan='2'>[[1tex]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TEX FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
|GENE= stf0 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 Mycobacterium smegmatis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900006:trehalose'>PRD_900006</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tex OCA], [https://pdbe.org/1tex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tex RCSB], [https://www.ebi.ac.uk/pdbsum/1tex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tex ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/P84151_MYCSM P84151_MYCSM]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tex_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tex ConSurf].
<div style="clear:both"></div>


'''Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose'''
==See Also==
 
*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Sulfolipid-1 (SL-1) is an abundant sulfated glycolipid and potential virulence factor found in Mycobacterium tuberculosis. SL-1 consists of a trehalose-2-sulfate (T2S) disaccharide elaborated with four lipids. We identified and characterized a conserved mycobacterial sulfotransferase, Stf0, which generates the T2S moiety of SL-1. Biochemical studies demonstrated that the enzyme requires unmodified trehalose as substrate and is sensitive to small structural perturbations of the disaccharide. Disruption of stf0 in Mycobacterium smegmatis and M. tuberculosis resulted in the loss of T2S and SL-1 formation, respectively. The structure of Stf0 at a resolution of 2.6 A reveals the molecular basis of trehalose recognition and a unique dimer configuration that encloses the substrate into a bipartite active site. These data provide strong evidence that Stf0 carries out the first committed step in the biosynthesis of SL-1 and establish a system for probing the role of SL-1 in M. tuberculosis infection.
[[Category: Large Structures]]
 
[[Category: Mycolicibacterium smegmatis]]
==About this Structure==
[[Category: Akey DL]]
1TEX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEX OCA].
[[Category: Berger JM]]
 
[[Category: Bertozzi CR]]
==Reference==
[[Category: Leary JA]]
Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis., Mougous JD, Petzold CJ, Senaratne RH, Lee DH, Akey DL, Lin FL, Munchel SE, Pratt MR, Riley LW, Leary JA, Berger JM, Bertozzi CR, Nat Struct Mol Biol. 2004 Aug;11(8):721-9. Epub 2004 Jul 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15258569 15258569]
[[Category: Lee DH]]
[[Category: Mycobacterium smegmatis]]
[[Category: Lin FL]]
[[Category: Single protein]]
[[Category: Mougous JD]]
[[Category: Akey, D L.]]
[[Category: Munchel SE]]
[[Category: Berger, J M.]]
[[Category: Petzold CJ]]
[[Category: Bertozzi, C R.]]
[[Category: Pratt MR]]
[[Category: Leary, J A.]]
[[Category: Riley LW]]
[[Category: Lee, D H.]]
[[Category: Senaratne RH]]
[[Category: Lin, F L.]]
[[Category: Mougous, J D.]]
[[Category: Munchel, S E.]]
[[Category: Petzold, C J.]]
[[Category: Pratt, M R.]]
[[Category: Riley, L W.]]
[[Category: Senaratne, R H.]]
[[Category: TRE]]
[[Category: mycobacterium]]
[[Category: sulfation]]
[[Category: sulfolipid]]
[[Category: sulfotransferase]]
[[Category: trehalose]]
[[Category: trehalose-2-sulfate]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:17:27 2008''

Latest revision as of 08:39, 14 February 2024

Mycobacterium smegmatis Stf0 Sulfotransferase with Trehalose

1tex, resolution 2.60Å

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