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==STRUCTURAL AND BIOCHEMICAL CHARACTERIZATION OF A NEW MAGNESIUM ION BINDING SITE NEAR TYR94 IN THE RESTRICTION ENDONUCLEASE PVUII==
 
<StructureSection load='1h56' size='340' side='right' caption='[[1h56]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
==Structural and biochemical characterization of a new magnesium ion binding site near Tyr94 in the restriction endonuclease PvuII==
<StructureSection load='1h56' size='340' side='right'caption='[[1h56]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1h56]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H56 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H56 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1h56]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_vulgaris Proteus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H56 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H56 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eyu|1eyu]], [[1f0o|1f0o]], [[1k0z|1k0z]], [[1ni0|1ni0]], [[1pvi|1pvi]], [[1pvu|1pvu]], [[2pvi|2pvi]], [[3pvi|3pvi]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h56 OCA], [https://pdbe.org/1h56 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h56 RCSB], [https://www.ebi.ac.uk/pdbsum/1h56 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h56 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h56 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1h56 RCSB], [http://www.ebi.ac.uk/pdbsum/1h56 PDBsum]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/T2P2_PROHU T2P2_PROHU] Recognizes the double-stranded sequence CAGCTG and cleaves after G-3.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 1h56" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Proteus vulgaris]]
[[Category: Proteus vulgaris]]
[[Category: Type II site-specific deoxyribonuclease]]
[[Category: Athanasiadis A]]
[[Category: Athanasiadis, A]]
[[Category: Jeltsch A]]
[[Category: Jeltsch, A]]
[[Category: Kokkinidis M]]
[[Category: Kokkinidis, M]]
[[Category: Lanio T]]
[[Category: Lanio, T]]
[[Category: Matzen C]]
[[Category: Matzen, C]]
[[Category: Pingoud A]]
[[Category: Pingoud, A]]
[[Category: Scheuring-Vanamee E]]
[[Category: Scheuring-Vanamee, E]]
[[Category: Simoncsits A]]
[[Category: Simoncsits, A]]
[[Category: Spyrida A]]
[[Category: Spyridaki, A]]
[[Category: Endonuclease]]
[[Category: Hydrolase]]
[[Category: Nuclease]]
[[Category: Type ii restriction endonuclease]]
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