4p6l: Difference between revisions

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==Crystal Structure of the Computationally Designed Transmembrane Metallotransporter in Octyl Glucoside==
==Crystal Structure of the Computationally Designed Transmembrane Metallotransporter in Octyl Glucoside==
<StructureSection load='4p6l' size='340' side='right' caption='[[4p6l]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='4p6l' size='340' side='right'caption='[[4p6l]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4p6l]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P6L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4P6L FirstGlance]. <br>
<table><tr><td colspan='2'>[[4p6l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4P6L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4P6L FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.803&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4p6j|4p6j]], [[4p6k|4p6k]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p6l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p6l RCSB], [http://www.ebi.ac.uk/pdbsum/4p6l PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4p6l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p6l OCA], [https://pdbe.org/4p6l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4p6l RCSB], [https://www.ebi.ac.uk/pdbsum/4p6l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4p6l ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The design of functional membrane proteins from first principles represents a grand challenge in chemistry and structural biology. Here, we report the design of a membrane-spanning, four-helical bundle that transports first-row transition metal ions Zn(2+) and Co(2+), but not Ca(2+), across membranes. The conduction path was designed to contain two di-metal binding sites that bind with negative cooperativity. X-ray crystallography and solid-state and solution nuclear magnetic resonance indicate that the overall helical bundle is formed from two tightly interacting pairs of helices, which form individual domains that interact weakly along a more dynamic interface. Vesicle flux experiments show that as Zn(2+) ions diffuse down their concentration gradients, protons are antiported. These experiments illustrate the feasibility of designing membrane proteins with predefined structural and dynamic properties.
De novo design of a transmembrane Zn(2)(+)-transporting four-helix bundle.,Joh NH, Wang T, Bhate MP, Acharya R, Wu Y, Grabe M, Hong M, Grigoryan G, DeGrado WF Science. 2014 Dec 19;346(6216):1520-4. doi: 10.1126/science.1261172. PMID:25525248<ref>PMID:25525248</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4p6l" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Acharya, R]]
[[Category: Large Structures]]
[[Category: DeGrado, W F]]
[[Category: Synthetic construct]]
[[Category: Joh, N H]]
[[Category: Acharya R]]
[[Category: De novo protein]]
[[Category: DeGrado WF]]
[[Category: De-novo designed]]
[[Category: Joh NH]]
[[Category: Transmembrane]]
[[Category: Transporter]]
Retrieved from "https://proteopedia.org/4p6l"