4rob: Difference between revisions

Latest revision as of 17:53, 20 September 2023

2.8A resolution structure of SRPN2 (K198C) from Anopheles gambiae

Structural highlights

4rob is a 3 chain structure with sequence from Anopheles gambiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SP2_ANOGA Serine protease inhibitor that functions in the melanization-mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine proteases CLIPB9 and CLIPB10, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656).^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

References

↑ Michel K, Budd A, Pinto S, Gibson TJ, Kafatos FC. Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei. EMBO Rep. 2005 Sep;6(9):891-7. PMID:16113656 doi:10.1038/sj.embor.7400478
↑ An C, Budd A, Kanost MR, Michel K. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-39. PMID:20953892 doi:10.1007/s00018-010-0543-z
↑ An C, Lovell S, Kanost MR, Battaile KP, Michel K. Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes. Proteins. 2011 Feb 3. doi: 10.1002/prot.23002. PMID:21465556 doi:10.1002/prot.23002
↑ Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K. Structural and Inhibitory Effects of Hinge Loop Mutagenesis in Serpin-2 from the Malaria Vector Anopheles gambiae. J Biol Chem. 2015 Jan 30;290(5):2946-56. doi: 10.1074/jbc.M114.625665. Epub 2014 , Dec 17. PMID:25525260 doi:https://dx.doi.org/10.1074/jbc.M114.625665
↑ Sousa GL, Bishnoi R, Baxter RHG, Povelones M. The CLIP-domain serine protease CLIPC9 regulates melanization downstream of SPCLIP1, CLIPA8, and CLIPA28 in the malaria vector Anopheles gambiae. PLoS Pathog. 2020 Oct 12;16(10):e1008985. PMID:33045027 doi:10.1371/journal.ppat.1008985
↑ Zhang X, Li M, El Moussawi L, Saab S, Zhang S, Osta MA, Michel K. CLIPB10 is a Terminal Protease in the Regulatory Network That Controls Melanization in the African Malaria Mosquito Anopheles gambiae. Front Cell Infect Microbiol. 2021 Jan 15;10:585986. PMID:33520733 doi:10.3389/fcimb.2020.585986

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OCA

[1] Michel K, Budd A, Pinto S, Gibson TJ, Kafatos FC. Anopheles gambiae SRPN2 facilitates midgut invasion by the malaria parasite Plasmodium berghei. EMBO Rep. 2005 Sep;6(9):891-7. PMID:16113656 doi:10.1038/sj.embor.7400478

[2] An C, Budd A, Kanost MR, Michel K. Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell Mol Life Sci. 2011 Jun;68(11):1929-39. PMID:20953892 doi:10.1007/s00018-010-0543-z

[3] An C, Lovell S, Kanost MR, Battaile KP, Michel K. Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes. Proteins. 2011 Feb 3. doi: 10.1002/prot.23002. PMID:21465556 doi:10.1002/prot.23002

[4] Zhang X, Meekins DA, An C, Zolkiewski M, Battaile KP, Kanost MR, Lovell S, Michel K. Structural and Inhibitory Effects of Hinge Loop Mutagenesis in Serpin-2 from the Malaria Vector Anopheles gambiae. J Biol Chem. 2015 Jan 30;290(5):2946-56. doi: 10.1074/jbc.M114.625665. Epub 2014 , Dec 17. PMID:25525260 doi:https://dx.doi.org/10.1074/jbc.M114.625665

[5] Sousa GL, Bishnoi R, Baxter RHG, Povelones M. The CLIP-domain serine protease CLIPC9 regulates melanization downstream of SPCLIP1, CLIPA8, and CLIPA28 in the malaria vector Anopheles gambiae. PLoS Pathog. 2020 Oct 12;16(10):e1008985. PMID:33045027 doi:10.1371/journal.ppat.1008985

[6] Zhang X, Li M, El Moussawi L, Saab S, Zhang S, Osta MA, Michel K. CLIPB10 is a Terminal Protease in the Regulatory Network That Controls Melanization in the African Malaria Mosquito Anopheles gambiae. Front Cell Infect Microbiol. 2021 Jan 15;10:585986. PMID:33520733 doi:10.3389/fcimb.2020.585986

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@@ Line 1: / Line 1: @@
 ==2.8A resolution structure of SRPN2 (K198C) from Anopheles gambiae==
-<StructureSection load='4rob' size='340' side='right' caption='[[4rob]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='4rob' size='340' side='right'caption='[[4rob]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4rob]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ROB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ROB FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4rob]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Anopheles_gambiae Anopheles gambiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ROB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ROB FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ro9|4ro9]], [[4roa|4roa]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rob OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rob RCSB], [http://www.ebi.ac.uk/pdbsum/4rob PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rob OCA], [https://pdbe.org/4rob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rob RCSB], [https://www.ebi.ac.uk/pdbsum/4rob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rob ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SP2_ANOGA SP2_ANOGA] Serine protease inhibitor that functions in the melanization-mediated immune response (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing the activation of phenoloxidases through the inhibiting of serine proteases CLIPB9 and CLIPB10, negatively regulates melanization in the hemolymph (PubMed:16113656, PubMed:20953892, PubMed:33520733, PubMed:33045027, PubMed:21465556, PubMed:25525260). By preventing melanization, has a detrimental role during P.berghei parasite mediated-infection and invasion of the mosquito midgut (PubMed:16113656).<ref>PMID:16113656</ref> <ref>PMID:20953892</ref> <ref>PMID:21465556</ref> <ref>PMID:25525260</ref> <ref>PMID:33045027</ref> <ref>PMID:33520733</ref>
+==See Also==
+*[[Serpin 3D structures|Serpin 3D structures]]
+== References ==
+<references/>
 __TOC__
 </StructureSection>
-[[Category: An, C]]
+[[Category: Anopheles gambiae]]
-[[Category: Battaile, K P]]
+[[Category: Large Structures]]
-[[Category: Lovell, S]]
+[[Category: An C]]
-[[Category: Meekins, D A]]
+[[Category: Battaile KP]]
-[[Category: Michel, K]]
+[[Category: Lovell S]]
-[[Category: Zhang, X]]
+[[Category: Meekins DA]]
-[[Category: Hydrolase inhibitor]]
+[[Category: Michel K]]
-[[Category: Insect immunity]]
+[[Category: Zhang X]]
-[[Category: Serine protease inhibitor]]
-[[Category: Serpin]]

4rob: Difference between revisions

Latest revision as of 17:53, 20 September 2023

2.8A resolution structure of SRPN2 (K198C) from Anopheles gambiae

Structural highlights

Function

See Also

References

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