2msf

NMR SOLUTION STRUCTURE OF SCORPION VENOM TOXIN Ts11 (TsPep1) FROM Tityus serrulatus

spinqualitylabelsall models 2msf Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2msf is a 1 chain structure with sequence from Tityus serrulatus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 15 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function KEX11_TITSE Potassium channel blocker. At 3 uM, this toxin blocks voltage-independently voltage-gated potassium channels rKv1.2/KCNA2 (25%), hKv1.3/KCNA3 (27%), rKv4.2/KCND2 (25%), Kv10.1/KCNH1/EAG1 (15%), Kv11/hERG (12%), and Shaker-IR (10%). On hKv1.3/KCNA3, the IC(50) is 17.1 +-3.3 uM. Publication Abstract from PubMed To date, several families of peptide toxins specifically interacting with ion channels in scorpion venom have been described. One of these families comprise peptide toxins (called KTxs), known to modulate potassium channels. Thus far, 202 KTxs have been reported, belonging to several subfamilies of KTxs (called alpha, beta, gamma, kappa, delta, and lambda-KTxs). Here we report on a previously described orphan toxin from Tityus serrulatus venom, named Ts11. We carried out an in-depth structure-function analysis combining 3D structure elucidation of Ts11 and electrophysiological characterization of the toxin. The Ts11 structure is highlighted by an Inhibitor Cystine Knot (ICK) type scaffold, completely devoid of the classical secondary structure elements (alpha-helix and/or beta-strand). This has, to the best of our knowledge, never been described before for scorpion toxins and therefore represents a novel, 6th type of structural fold for these scorpion peptides. On the basis of their preferred interaction with voltage-gated K channels, as compared to all the other targets tested, it can be postulated that Ts11 is the first member of a new subfamily, designated as epsilon-KTx. Structural and Functional Elucidation of Peptide Ts11 Shows Evidence of a Novel Subfamily of Scorpion Venom Toxins.,Cremonez CM, Maiti M, Peigneur S, Cassoli JS, Dutra AA, Waelkens E, Lescrinier E, Herdewijn P, de Lima ME, Pimenta AM, Arantes EC, Tytgat J Toxins (Basel). 2016 Sep 30;8(10). pii: E288. PMID:27706049 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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