4eza: Difference between revisions

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==Crystal structure of the atypical phosphoinositide (aPI) binding domain of IQGAP2==
==Crystal structure of the atypical phosphoinositide (aPI) binding domain of IQGAP2==
<StructureSection load='4eza' size='340' side='right' caption='[[4eza]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='4eza' size='340' side='right'caption='[[4eza]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4eza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EZA FirstGlance]. <br>
<table><tr><td colspan='2'>[[4eza]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EZA FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IQGAP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eza OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eza RCSB], [http://www.ebi.ac.uk/pdbsum/4eza PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eza OCA], [https://pdbe.org/4eza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eza RCSB], [https://www.ebi.ac.uk/pdbsum/4eza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eza ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/IQGA2_HUMAN IQGA2_HUMAN]] Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.  
[https://www.uniprot.org/uniprot/IQGA2_HUMAN IQGA2_HUMAN] Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Class I phosphoinositide (PI) 3-kinases act through effector proteins whose 3-PI selectivity is mediated by a limited repertoire of structurally defined, lipid recognition domains. We describe here the lipid preferences and crystal structure of a new class of PI binding module exemplified by select IQGAPs (IQ motif containing GTPase activating proteins) known to co-ordinate cellular signalling events and cytoskeletal dynamics. This module is defined by a C-terminal 105-107 amino acid region of which that from IQGAP1 and 2, but not IQGAP3, binds preferentially to phosphatidylinositol 3,4,5-trisphosphate (PtdInsP(3)). The binding affinity for PtdInsP(3), together with other, secondary target-recognition characteristics, are comparable to those of the pleckstrin homology (PH) domain of cytohesin-3 (general receptor for phosphoinositides 1), an established PtdInsP(3) effector protein. Importantly, the IQGAP1 C-terminal domain and the cytohesin-3 PH domain, each tagged with enhanced green fluorescent protein, both re-localized from the cytosol to the cell periphery following the activation of PI 3-kinase in Swiss 3T3 fibroblasts, consistent with their common, selective recognition of endogenous 3-PI(s). The crystal structure of the C-terminal IQGAP2 PI binding module reveals unexpected topological similarity to an integral fold of C2 domains, including a putative basic binding pocket. We propose that this module integrates select IQGAP proteins with PI 3-kinase signalling and constitutes a novel, atypical phosphoinositide (aPI) binding domain that may represent the first of a larger group, each perhaps structurally unique but collectively dissimilar from the known PI recognition modules.
 
IQGAP proteins reveal an atypical phosphoinositide (aPI) binding domain with a pseudo C2 domain fold.,Dixon MJ, Gray A, Schenning M, Agacan M, Tempel W, Tong Y, Nedyalkova L, Park HW, Leslie NR, van Aalten DM, Downes CP, Batty IH J Biol Chem. 2012 Apr 5. PMID:22493426<ref>PMID:22493426</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Aalten, D M.F Van]]
[[Category: Large Structures]]
[[Category: Agacan, M]]
[[Category: Agacan M]]
[[Category: Arrowsmith, C H]]
[[Category: Arrowsmith CH]]
[[Category: Batty, I H]]
[[Category: Batty IH]]
[[Category: Bochkarev, A]]
[[Category: Bochkarev A]]
[[Category: Bountra, C]]
[[Category: Bountra C]]
[[Category: Crombet, L]]
[[Category: Crombet L]]
[[Category: Dixon, M J]]
[[Category: Dixon MJ]]
[[Category: Downes, C P]]
[[Category: Downes CP]]
[[Category: Edwards, A M]]
[[Category: Edwards AM]]
[[Category: Gray, A]]
[[Category: Gray A]]
[[Category: Leslie, N R]]
[[Category: Leslie NR]]
[[Category: Nedyalkova, L]]
[[Category: Nedyalkova L]]
[[Category: Park, H]]
[[Category: Park H]]
[[Category: Structural genomic]]
[[Category: Schenning M]]
[[Category: Schenning, M]]
[[Category: Tempel W]]
[[Category: Tempel, W]]
[[Category: Tong Y]]
[[Category: Tong, Y]]
[[Category: Van Aalten DMF]]
[[Category: Weigelt, J]]
[[Category: Weigelt J]]
[[Category: Zhong, N]]
[[Category: Zhong N]]
[[Category: Gap]]
[[Category: Sgc]]
[[Category: Signaling protein]]
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