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'''Unreleased structure'''


The entry 4pex is ON HOLD  until Paper Publication
==Structure of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with glucose==
<StructureSection load='4pex' size='340' side='right'caption='[[4pex]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pex]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._SirexAA-E Streptomyces sp. SirexAA-E]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PEX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pex OCA], [https://pdbe.org/4pex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pex RCSB], [https://www.ebi.ac.uk/pdbsum/4pex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pex ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LAM55_STREK LAM55_STREK] Exo-beta-1,3-glucanase that specifically hydrolyzes laminarin and laminarioligosaccharides, producing glucose and laminaribiose as end products.<ref>PMID:25752603</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Carbohydrate Active Enzyme (CaZY) database indicates that glycoside hydrolase family 55 (GH55) contains both endo- and exo-beta -1,3-glucanases. The founding structure of the GH55 is PcLam55A from the white-rot fungus Phanaerochaete chrysosporium (Ishida, T., et al. (2009) J. Biol. Chem. 284, 10100-10109). Here, we present high resolution crystal structures of bacterial SacteLam55A from the highly cellulolytic Streptomyces sp. SirexAA-E with bound substrates and product. These structures, along with mutagenesis and kinetic studies implicate Glu502 as the catalytic acid (as proposed earlier for Glu663 in PcLam55A) and a proton relay network of four residues in activating water as the nucleophile. Further, a set of conserved aromatic residues that define the active site apparently enforce an exo-glucanase reactivity as demonstrated by exhaustive hydrolysis reactions with purified laminarioligosaccharides. Two additional aromatic residues that line the substrate-binding channel show substrate-dependent conformational flexibility that may promote processive reactivity of the bound oligosaccharide in the bacterial enzymes. Gene synthesis carried out on ~30% of the GH55 family gave 34 active enzymes (19% functional coverage of the non-redundant members of GH55). These active enzymes reacted with only laminarin from a panel of 10 different soluble and insoluble polysaccharides and displayed a broad range of specific activities, and optima for pH and temperature. Application of this experimental method provides a new, systematic way to annotate GH phylogenetic space for functional properties.


Authors: Bianchetti, C.M., Takasuka, T.E., Yik, E.J., Bergeman, L.F., Fox, B.G.
Active site and laminarin binding in glycoside hydrolase family 55.,Bianchetti CM, Takasuka TE, Deutsch S, Udell HS, Yik EJ, Bergeman LF, Fox BG J Biol Chem. 2015 Mar 9. pii: jbc.M114.623579. PMID:25752603<ref>PMID:25752603</ref>


Description: Structure of the E502A variant of sacteLam55A from Streptomyces sp. SirexAA-E in complex with glucose
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Fox, B.G]]
<div class="pdbe-citations 4pex" style="background-color:#fffaf0;"></div>
[[Category: Takasuka, T.E]]
== References ==
[[Category: Bianchetti, C.M]]
<references/>
[[Category: Yik, E.J]]
__TOC__
[[Category: Bergeman, L.F]]
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces sp. SirexAA-E]]
[[Category: Bergeman LF]]
[[Category: Bianchetti CM]]
[[Category: Fox BG]]
[[Category: Takasuka TE]]
[[Category: Yik EJ]]
Retrieved from "https://proteopedia.org/4pex"