4x33: Difference between revisions

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'''Unreleased structure'''


The entry 4x33 is ON HOLD  until Paper Publication
==Structure of the Elongator cofactor complex Kti11/Kti13 at 1.45A==
<StructureSection load='4x33' size='340' side='right'caption='[[4x33]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4x33]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X33 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=DXE:1,2-DIMETHOXYETHANE'>DXE</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x33 OCA], [https://pdbe.org/4x33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x33 RCSB], [https://www.ebi.ac.uk/pdbsum/4x33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x33 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DPH3_YEAST DPH3_YEAST] Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2. Required for conferring sensitivity to K.lactis zymocin.<ref>PMID:15485916</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Modification of wobble uridines of many eukaryotic tRNAs requires the Elongator complex, a highly conserved six-subunit eukaryotic protein assembly, as well as the Killer toxin-insensitive (Kti) proteins 11-14. Kti11 was additionally shown to be implicated in the biosynthesis of diphthamide, a post-translationally modified histidine of translation elongation factor 2. Recent data indicate that iron-bearing Kti11 functions as an electron donor to the [4Fe-4S] cluster of radical S-Adenosylmethionine enzymes, triggering the subsequent radical reaction. We show here that recombinant yeast Kti11 forms a stable 1 : 1 complex with Kti13. To obtain insights into the function of this heterodimer, the Kti11/Kti13 complex was purified to homogeneity, crystallized, and its structure determined at 1.45 A resolution. The importance of several residues mediating complex formation was confirmed by mutagenesis. Kti13 adopts a fold characteristic of RCC1-like proteins. The seven-bladed beta-propeller consists of a unique mixture of four- and three-stranded blades. In the complex, Kti13 orients Kti11 and restricts access to its electron-carrying iron atom, constraining the electron transfer capacity of Kti11. Based on these findings, we propose a role for Kti13, and discuss the possible functional implications of complex formation. DATABASE: Structural data have been submitted to the Protein Data Bank under accession number 4X33.


Authors: Kolaj-Robin, O., McEwen, A.G., Cavarelli, J., Seraphin, B.
Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification.,Kolaj-Robin O, McEwen AG, Cavarelli J, Seraphin B FEBS J. 2015 Jan 21. doi: 10.1111/febs.13199. PMID:25604895<ref>PMID:25604895</ref>


Description: Structure of the Elongator cofactor complex Kti11/Kti13 at 1.45A
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Cavarelli, J]]
<div class="pdbe-citations 4x33" style="background-color:#fffaf0;"></div>
[[Category: Mcewen, A.G]]
== References ==
[[Category: Kolaj-Robin, O]]
<references/>
[[Category: Seraphin, B]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Cavarelli J]]
[[Category: Kolaj-Robin O]]
[[Category: McEwen AG]]
[[Category: Seraphin B]]
Retrieved from "https://proteopedia.org/4x33"