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| [[Image:2a7e.gif|left|200px]]
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| {{Structure
| | ==On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength== |
| |PDB= 2a7e |SIZE=350|CAPTION= <scene name='initialview01'>2a7e</scene>, resolution 1.66Å
| | <StructureSection load='2a7e' size='340' side='right'caption='[[2a7e]], [[Resolution|resolution]] 1.66Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[2a7e]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A7E FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a7e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a7e OCA], [https://pdbe.org/2a7e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a7e RCSB], [https://www.ebi.ac.uk/pdbsum/2a7e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a7e ProSAT]</span></td></tr> |
| }}
| | </table> |
| | | __TOC__ |
| '''On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength'''
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| | | [[Category: Mueller-Dieckmann C]] |
| ==Overview== | | [[Category: Panjikar S]] |
| Complete and highly redundant data sets were collected at different wavelengths between 0.80 and 2.65 A for a total of ten different protein and DNA model systems. The magnitude of the anomalous signal-to-noise ratio as assessed by the quotient R(anom)/R(r.i.m.) was found to be influenced by the data-collection wavelength and the nature of the anomalously scattering substructure. By utilizing simple empirical correlations, for instance between the estimated deltaF/F and the expected R(anom) or the data-collection wavelength and the expected R(r.i.m.), the wavelength at which the highest anomalous signal-to-noise ratio can be expected could be estimated even before the experiment. Almost independent of the nature of the anomalously scattering substructure and provided that no elemental X-ray absorption edge is nearby, this optimal wavelength is 2.1 A.
| | [[Category: Tucker PA]] |
| | | [[Category: Weiss MS]] |
| ==About this Structure== | |
| 2A7E is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A7E OCA].
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| ==Reference==
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| On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength., Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1263-72. Epub 2005, Aug 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16131760 16131760]
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| [[Category: Protein complex]] | |
| [[Category: Mueller-Dieckmann, C.]] | |
| [[Category: Panjikar, S.]] | |
| [[Category: Tucker, P A.]] | |
| [[Category: Weiss, M S.]] | |
| [[Category: a-dna]]
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| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:46:16 2008''
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