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==Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')==
==Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')==
<StructureSection load='4aag' size='340' side='right' caption='[[4aag]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
<StructureSection load='4aag' size='340' side='right'caption='[[4aag]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4aag]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AAG FirstGlance]. <br>
<table><tr><td colspan='2'>[[4aag]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AAG FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4aaf|4aaf]], [[1g9z|1g9z]], [[1bp7|1bp7]], [[2vbl|2vbl]], [[4aae|4aae]], [[2vbo|2vbo]], [[1mow|1mow]], [[1t9i|1t9i]], [[4aad|4aad]], [[1u0d|1u0d]], [[1t9j|1t9j]], [[1n3e|1n3e]], [[1af5|1af5]], [[4aab|4aab]], [[2vbj|2vbj]], [[1g9y|1g9y]], [[2vbn|2vbn]], [[1n3f|1n3f]], [[1u0c|1u0c]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4aag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aag OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4aag RCSB], [http://www.ebi.ac.uk/pdbsum/4aag PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4aag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4aag OCA], [https://pdbe.org/4aag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4aag RCSB], [https://www.ebi.ac.uk/pdbsum/4aag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4aag ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE]] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.  
[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 4aag" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Endonuclease|Endonuclease]]
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Abramo, M D]]
[[Category: Large Structures]]
[[Category: Duchateau, P]]
[[Category: Synthetic construct]]
[[Category: Epinat, J C]]
[[Category: D'Abramo M]]
[[Category: Gervasio, F]]
[[Category: Duchateau P]]
[[Category: Grizot, S]]
[[Category: Epinat JC]]
[[Category: Marenchino, M]]
[[Category: Gervasio F]]
[[Category: Molina, R]]
[[Category: Grizot S]]
[[Category: Montoya, G]]
[[Category: Marenchino M]]
[[Category: Prieto, J]]
[[Category: Molina R]]
[[Category: Redondo, P]]
[[Category: Montoya G]]
[[Category: Stella, S]]
[[Category: Prieto J]]
[[Category: Valton, J]]
[[Category: Redondo P]]
[[Category: Gene targeting]]
[[Category: Stella S]]
[[Category: Homing endonuclease]]
[[Category: Valton J]]
[[Category: Hydrolase-dna complex]]
[[Category: Protein-dna interaction]]