3qq2

Crystal Structure of the Beta Domain of the Bordetella Autotransporter Brka

spinqualitylabelsall models 3qq2, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3qq2 is a 3 chain structure with sequence from Bordetella pertussis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function BRKA_BORPE Inhibits the classical pathway of complement activation and prevents accumulation of deposited C4. Publication Abstract from PubMed Whooping cough (pertussis) is a highly contagious acute respiratory illness of humans caused by the Gram-negative bacterial pathogen Bordetella pertussis. The AT (autotransporter) BrkA (Bordetella serum-resistance killing protein A) is an important B. pertussis virulence factor that confers serum resistance and mediates adherence. In the present study, we have solved the crystal structure of the BrkA beta-domain at 3 A (1 A=0.1 nm) resolution. Special features are a hairpin-like structure formed by the external loop L4, which is observed fortuitously sitting inside the pore of the crystallographic adjacent beta-domain, and a previously undiscovered hydrophobic cavity formed by patches on loop L4 and beta-strands S5 and S6. This adopts a ubiquitous structure characteristic of all AT beta-domains. Mutagenesis studies have demonstrated that the hairpin-like structure and hydrophobic cavity are crucial for BrkA passenger domain (virulence effector) translocation. This structure helps in understanding the molecular mechanism of AT assembly and secretion and provides a potential target for anti-pertussis drug design. Autotransporter passenger domain secretion requires a hydrophobic cavity at the extracellular entrance of the beta-domain pore.,Zhai Y, Zhang K, Huo Y, Zhu Y, Zhou Q, Lu J, Black I, Pang X, Roszak AW, Zhang X, Isaacs NW, Sun F Biochem J. 2011 May 1;435(3):577-87. PMID:21306302 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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