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==Crystal structure of actin in complex with lobophorolide==
==Crystal structure of actin in complex with lobophorolide==
<StructureSection load='3m6g' size='340' side='right' caption='[[3m6g]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='3m6g' size='340' side='right'caption='[[3m6g]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3m6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobophora_variegata Lobophora variegata] and [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M6G FirstGlance]. <br>
<table><tr><td colspan='2'>[[3m6g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3M6G FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LO3:(1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-HYDROXY-9-{(1S,2S,3S)-2-HYDROXY-5-[(2S,4R,6S)-4-METHOXY-6-METHYLTETRAHYDRO-2H-PYRAN-2-YL]-1,3-DIMETHYLPENTYL}-3,5,7,17-TETRAMETHOXY-8,14-DIMETHYL-11H-SPIRO[10,23-DIOXABICYCLO[17.3.1]TRICOSA-12,14,20-TRIENE-4,2-OXIRAN]-11-ONE'>LO3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1yxq|1yxq]], [[2vyp|2vyp]], [[2asm|2asm]], [[2aso|2aso]], [[2asp|2asp]], [[1qz5|1qz5]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=LO3:(1S,3S,4S,5S,7R,8S,9R,12E,14E,16R,17R,19R)-16-HYDROXY-9-{(1S,2S,3S)-2-HYDROXY-5-[(2S,4R,6S)-4-METHOXY-6-METHYLTETRAHYDRO-2H-PYRAN-2-YL]-1,3-DIMETHYLPENTYL}-3,5,7,17-TETRAMETHOXY-8,14-DIMETHYL-11H-SPIRO[10,23-DIOXABICYCLO[17.3.1]TRICOSA-12,14,20-TRIENE-4,2-OXIRAN]-11-ONE'>LO3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m6g RCSB], [http://www.ebi.ac.uk/pdbsum/3m6g PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3m6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m6g OCA], [https://pdbe.org/3m6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3m6g RCSB], [https://www.ebi.ac.uk/pdbsum/3m6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3m6g ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT]] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.  
[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Actin filament-disrupting marine macrolides are promising templates from which to design therapeutics against cancer and other diseases that co-opt the actin cytoskeleton. Typically, these macrolides form either a 1:1 or 2:1 actin-macrolide complex where their aliphatic side chain, or "tail," has been reported to convey the major determinant of cytotoxicity. We now report the structure of the marine macrolide lobophorolide bound to actin with a unique 2:2 stoichiometry in which two lobophorolide molecules cooperate to form a dimerization interface that is composed entirely of the macrolide "ring" region, and each molecule of lobophorolide interacts with both actin subunits via their ring and tail regions to tether the subunits together. This binding mode imposes multiple barriers against microfilament stability and holds important implications for development of actin-targeting drugs and the evolution of macrolide biosynthetic enzymes.
 
Two molecules of lobophorolide cooperate to stabilize an actin dimer using both their "ring" and "tail" region.,Blain JC, Mok YF, Kubanek J, Allingham JS Chem Biol. 2010 Aug 27;17(8):802-7. PMID:20797609<ref>PMID:20797609</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Actin|Actin]]
*[[Actin 3D structures|Actin 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Lobophora variegata]]
[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
[[Category: Allingham, J S]]
[[Category: Allingham JS]]
[[Category: Actin]]
[[Category: Actin dimer]]
[[Category: Atp-binding]]
[[Category: Cytoskeleton]]
[[Category: Lobophorolide]]
[[Category: Macrolide]]
[[Category: Marine toxin]]
[[Category: Muscle protein]]
[[Category: Nucleotide-binding]]
[[Category: Structural protein]]
Retrieved from "https://proteopedia.org/3m6g"