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==MthK channel pore T59A mutant==
==MthK channel pore T59A mutant==
<StructureSection load='3ous' size='340' side='right' caption='[[3ous]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
<StructureSection load='3ous' size='340' side='right'caption='[[3ous]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3ous]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OUS FirstGlance]. <br>
<table><tr><td colspan='2'>[[3ous]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OUS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ous FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ous OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ous RCSB], [http://www.ebi.ac.uk/pdbsum/3ous PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ous FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ous OCA], [https://pdbe.org/3ous PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ous RCSB], [https://www.ebi.ac.uk/pdbsum/3ous PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ous ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.  
[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Selective ion conduction across ion channel pores is central to cellular physiology. To understand the underlying principles of ion selectivity in tetrameric cation channels, we engineered a set of cation channel pores based on the nonselective NaK channel and determined their structures to high resolution. These structures showcase an ensemble of selectivity filters with a various number of contiguous ion binding sites ranging from 2 to 4, with each individual site maintaining a geometry and ligand environment virtually identical to that of equivalent sites in K(+) channel selectivity filters. Combined with single channel electrophysiology, we show that only the channel with four ion binding sites is K(+) selective, whereas those with two or three are nonselective and permeate Na(+) and K(+) equally well. These observations strongly suggest that the number of contiguous ion binding sites in a single file is the key determinant of the channel's selectivity properties and the presence of four sites in K(+) channels is essential for highly selective and efficient permeation of K(+) ions.
 
Tuning the ion selectivity of tetrameric cation channels by changing the number of ion binding sites.,Derebe MG, Sauer DB, Zeng W, Alam A, Shi N, Jiang Y Proc Natl Acad Sci U S A. 2010 Dec 27. PMID:21187421<ref>PMID:21187421</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
*[[Potassium Channel|Potassium Channel]]
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Methanothermobacter thermautotrophicus]]
[[Category: Large Structures]]
[[Category: Alam, A]]
[[Category: Methanothermobacter thermautotrophicus str. Delta H]]
[[Category: Derebe, M G]]
[[Category: Alam A]]
[[Category: Jiang, Y]]
[[Category: Derebe MG]]
[[Category: Sauer, D B]]
[[Category: Jiang Y]]
[[Category: Shi, N]]
[[Category: Sauer DB]]
[[Category: Zeng, W]]
[[Category: Shi N]]
[[Category: Ion channel]]
[[Category: Zeng W]]
[[Category: Membrane]]
[[Category: Membrane protein]]

Latest revision as of 10:36, 21 February 2024

MthK channel pore T59A mutant

3ous, resolution 1.75Å

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