2fqm: Difference between revisions

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[[Image:2fqm.gif|left|200px]]


{{Structure
==Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus==
|PDB= 2fqm |SIZE=350|CAPTION= <scene name='initialview01'>2fqm</scene>, resolution 2.3&Aring;
<StructureSection load='2fqm' size='340' side='right'caption='[[2fqm]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND=
<table><tr><td colspan='2'>[[2fqm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FQM FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
|GENE= P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11277 Vesicular stomatitis Indiana virus])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2fqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fqm OCA], [https://pdbe.org/2fqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2fqm RCSB], [https://www.ebi.ac.uk/pdbsum/2fqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2fqm ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus'''
[https://www.uniprot.org/uniprot/PHOSP_VSIVM PHOSP_VSIVM] Essential component of the RNA polymerase transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. May act as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly (By similarity).
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
In the replication cycle of nonsegmented negative-strand RNA viruses, the viral RNA-dependent RNA polymerase (L) recognizes a nucleoprotein (N)-enwrapped RNA template during the RNA polymerase reaction. The viral phosphoprotein (P) is a polymerase cofactor essential for this recognition. We report here the 2.3-angstroms-resolution crystal structure of the central domain (residues 107 to 177) of P from vesicular stomatitis virus. The fold of this domain consists of a beta hairpin, an alpha helix, and another beta hairpin. The alpha helix provides the stabilizing force for forming a homodimer, while the two beta hairpins add additional stabilization by forming a four-stranded beta sheet through domain swapping between two molecules. This central dimer positions the N- and C-terminal domains of P to interact with the N and L proteins, allowing the L protein to specifically recognize the nucleocapsid-RNA template and to progress along the template while concomitantly assembling N with nascent RNA. The interdimer interactions observed in the noncrystallographic packing may offer insight into the mechanism of the RNA polymerase processive reaction along the viral nucleocapsid-RNA template.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fq/2fqm_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
2FQM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FQM OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2fqm ConSurf].
Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus., Ding H, Green TJ, Lu S, Luo M, J Virol. 2006 Mar;80(6):2808-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16501089 16501089]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Vesicular stomatitis indiana virus]]
</StructureSection>
[[Category: Ding, H.]]
[[Category: Large Structures]]
[[Category: Green, T J.]]
[[Category: Vesicular stomatitis Indiana virus]]
[[Category: Lu, S.]]
[[Category: Ding H]]
[[Category: Luo, M.]]
[[Category: Green TJ]]
[[Category: cofactor]]
[[Category: Lu S]]
[[Category: negative strand rna virus]]
[[Category: Luo M]]
[[Category: polymerase]]
[[Category: replication]]
 
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Retrieved from "https://proteopedia.org/2fqm"