2ftx: Difference between revisions

Latest revision as of 15:33, 3 March 2021

Crystal structure of the yeast kinetochore Spc24/Spc25 globular domain

Structural highlights

2ftx is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	NA, PO4
NonStd Res:	MSE
Gene:	YER018C (ATCC 18824), YMR117C, YM9718.16C (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.^[1] ^[2] [SPC24_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451
↑ McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:https://dx.doi.org/10.1101/gad.1040903
↑ Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451
↑ Le Masson I, Saveanu C, Chevalier A, Namane A, Gobin R, Fromont-Racine M, Jacquier A, Mann C. Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication. Mol Microbiol. 2002 Mar;43(6):1431-43. PMID:11952896
↑ McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:https://dx.doi.org/10.1101/gad.1040903

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451

[2] McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:https://dx.doi.org/10.1101/gad.1040903

[3] Wigge PA, Kilmartin JV. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001 Jan 22;152(2):349-60. PMID:11266451

[4] Le Masson I, Saveanu C, Chevalier A, Namane A, Gobin R, Fromont-Racine M, Jacquier A, Mann C. Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication. Mol Microbiol. 2002 Mar;43(6):1431-43. PMID:11952896

[5] McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ, Stukenberg PT. The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity. Genes Dev. 2003 Jan 1;17(1):101-14. PMID:12514103 doi:https://dx.doi.org/10.1101/gad.1040903

[1]

[2]

[3]

[4]

[5]

@@ Line 1: / Line 1: @@
-[[Image:2ftx.gif|left|200px]]
- {{Structure
+==Crystal structure of the yeast kinetochore Spc24/Spc25 globular domain==
-|PDB= 2ftx |SIZE=350|CAPTION= <scene name='initialview01'>2ftx</scene>, resolution 1.90&Aring;
+<StructureSection load='2ftx' size='340' side='right'caption='[[2ftx]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
+<table><tr><td colspan='2'>[[2ftx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FTX FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-|GENE= YER018C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), YMR117C, YM9718.16C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-}}
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YER018C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), YMR117C, YM9718.16C ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ftx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ftx OCA], [https://pdbe.org/2ftx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ftx RCSB], [https://www.ebi.ac.uk/pdbsum/2ftx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ftx ProSAT]</span></td></tr>
-'''Crystal structure of the yeast kinetochore Spc24/Spc25 globular domain'''
+</table>
+== Function ==
+[[https://www.uniprot.org/uniprot/SPC25_YEAST SPC25_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:12514103</ref>  [[https://www.uniprot.org/uniprot/SPC24_YEAST SPC24_YEAST]] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.<ref>PMID:11266451</ref> <ref>PMID:11952896</ref> <ref>PMID:12514103</ref>
-==About this Structure==
+== Evolutionary Conservation ==
-FTX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FTX OCA].
+[[Image:Consurf_key_small.gif|200px|right]]
-[[Category: Protein complex]]
+Check<jmol>
-[[Category: Saccharomyces cerevisiae]]
+  <jmolCheckbox>
-[[Category: Harrison, S C.]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ft/2ftx_consurf.spt"</scriptWhenChecked>
-[[Category: Wei, R R.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: NA]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: PO4]]
+  </jmolCheckbox>
-[[Category: alpha-beta]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ftx ConSurf].
-[[Category: coiled-coil]]
+<div style="clear:both"></div>
-[[Category: complex]]
+== References ==
+<references/>
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:56:41 2008''
+__TOC__
+</StructureSection>
+[[Category: Atcc 18824]]
+[[Category: Large Structures]]
+[[Category: Harrison, S C]]
+[[Category: Wei, R R]]
+[[Category: Alpha-beta]]
+[[Category: Coiled-coil]]
+[[Category: Complex]]
+[[Category: Protein binding]]
+[[Category: Structural protein]]

2ftx: Difference between revisions

Latest revision as of 15:33, 3 March 2021

Crystal structure of the yeast kinetochore Spc24/Spc25 globular domain

Structural highlights

Function

Evolutionary Conservation

References

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