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==Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form==
==Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form==
<StructureSection load='3oti' size='340' side='right' caption='[[3oti]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='3oti' size='340' side='right'caption='[[3oti]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3oti]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OTI FirstGlance]. <br>
<table><tr><td colspan='2'>[[3oti]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Micromonospora_echinospora Micromonospora echinospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OTI FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C0T:CALICHEAMICIN+T0'>C0T</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.597&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3d0r|3d0r]], [[3d0q|3d0q]], [[3otg|3otg]], [[3oth|3oth]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C0T:CALICHEAMICIN+T0'>C0T</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TYD:THYMIDINE-5-DIPHOSPHATE'>TYD</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">calG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1877 Micromonospora echinospora])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oti OCA], [https://pdbe.org/3oti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oti RCSB], [https://www.ebi.ac.uk/pdbsum/3oti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oti ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oti OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oti RCSB], [http://www.ebi.ac.uk/pdbsum/3oti PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q8KND7_MICEC Q8KND7_MICEC]
Glycosyltransferases are useful synthetic catalysts for generating natural products with sugar moieties. Although several natural product glycosyltransferase structures have been reported, design principles of glycosyltransferase engineering for the generation of glycodiversified natural products has fallen short of its promise, partly due to a lack of understanding of the relationship between structure and function. Here, we report structures of all four calicheamicin glycosyltransferases (CalG1, CalG2, CalG3, and CalG4), whose catalytic functions are clearly regiospecific. Comparison of these four structures reveals a conserved sugar donor binding motif and the principles of acceptor binding region reshaping. Among them, CalG2 possesses a unique catalytic motif for glycosylation of hydroxylamine. Multiple glycosyltransferase structures in a single natural product biosynthetic pathway are a valuable resource for understanding regiospecific reactions and substrate selectivities and will help future glycosyltransferase engineering.
 
Complete set of glycosyltransferase structures in the calicheamicin biosynthetic pathway reveals the origin of regiospecificity.,Chang A, Singh S, Helmich KE, Goff RD, Bingman CA, Thorson JS, Phillips GN Jr Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17649-54. Epub 2011 Oct 10. PMID:21987796<ref>PMID:21987796</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Micromonospora echinospora]]
[[Category: Micromonospora echinospora]]
[[Category: Bingman, C A]]
[[Category: Bingman CA]]
[[Category: Structural genomic]]
[[Category: Chang A]]
[[Category: Chang, A]]
[[Category: Phillips Jr GN]]
[[Category: NatPro, Enzyme Discovery for Natural Product Biosynthesis]]
[[Category: Singh S]]
[[Category: Phillips, G N]]
[[Category: Thorson JS]]
[[Category: Singh, S]]
[[Category: Thorson, J S]]
[[Category: Calicheamicin]]
[[Category: Cesg]]
[[Category: Glycosyltransferase]]
[[Category: Gt-b fold]]
[[Category: PSI, Protein structure initiative]]
[[Category: Tdp]]
[[Category: Transferase-antibiotic complex]]

Latest revision as of 10:36, 21 February 2024

Crystal Structure of CalG3, Calicheamicin Glycostyltransferase, TDP and calicheamicin T0 bound form

3oti, resolution 1.60Å

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