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==CRYSTAL STRUCTURE OF R(GUGUGUA)D(C) WITH TANDEM G-U/U-G WOBBLE PAIRS WITH STRAND SLIPPAGE==
==CRYSTAL STRUCTURE OF R(GUGUGUA)D(C) WITH TANDEM G-U/U-G WOBBLE PAIRS WITH STRAND SLIPPAGE==
<StructureSection load='332d' size='340' side='right' caption='[[332d]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
<StructureSection load='332d' size='340' side='right'caption='[[332d]], [[Resolution|resolution]] 1.58&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[332d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=332D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=332D FirstGlance]. <br>
<table><tr><td colspan='2'>[[332d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=332D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=332D FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=332d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=332d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=332d RCSB], [http://www.ebi.ac.uk/pdbsum/332d PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.58&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=332d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=332d OCA], [https://pdbe.org/332d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=332d RCSB], [https://www.ebi.ac.uk/pdbsum/332d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=332d ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
To better understand the frequent occurrence of adjacent wobble pairs in ribosomal RNAs we have determined the crystal structure of the RNA duplex, r(GUGUGUA)dC with the 3'-terminal deoxy C residue. Two different crystal forms of the duplex were obtained and both belong to the rhombohedral space group, R3. Crystal form I has hexagonal unit cell dimensions, a = b = 40.82 A and c = 66.09 A and diffracts to 1.58 A resolution, while crystal form II has a = b = 47.11 A and c = 59.86 A, diffracting only to 2.50 A resolution. Both structures were solved by the molecular replacement method using different starting models. In spite of the large differences in the cell dimensions the overall structures in both crystals are similar. Instead of the expected blunt-end duplex with four consecutive G x U pairs, the slippage of the strands resulted in two different tandem G x U/U x G wobble pairs involving two of the central and two of the 5' overhang bases, still yielding a total of four wobble pairs. These tandem wobble pairs are flanked by two Watson-Crick pairs. The A-type duplexes stack in the familiar head-to-tail fashion forming a pseudocontinuous helix. The wobble pairs of the present motif II (G x U/U x G) structure stack with a low twist angle of 25.3 degrees in contrast to that of motif I (U x G/G x U), 38.1 degrees. The four wobble pairs are characteristically heavily hydrated in both the grooves accounting for their stability.
Crystal structure of r(GUGUGUA)dC with tandem G x U/U x G wobble pairs with strand slippage.,Biswas R, Sundaralingam M J Mol Biol. 1997 Jul 18;270(3):511-9. PMID:9237915<ref>PMID:9237915</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Biswas, R]]
[[Category: Large Structures]]
[[Category: Sundaralingam, M]]
[[Category: Biswas R]]
[[Category: A-dna/rna]]
[[Category: Sundaralingam M]]
[[Category: Dna-rna complex]]
[[Category: Double helix]]
[[Category: Overhanging base]]

Latest revision as of 08:39, 7 February 2024

CRYSTAL STRUCTURE OF R(GUGUGUA)D(C) WITH TANDEM G-U/U-G WOBBLE PAIRS WITH STRAND SLIPPAGE

332d, resolution 1.58Å

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