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==Structure of the C-terminal Domain of Transcription Factor IIB from Trypanosoma brucei==
==Structure of the C-terminal Domain of Transcription Factor IIB from Trypanosoma brucei==
<StructureSection load='3h4c' size='340' side='right' caption='[[3h4c]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='3h4c' size='340' side='right'caption='[[3h4c]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3h4c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3H4C FirstGlance]. <br>
<table><tr><td colspan='2'>[[3h4c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H4C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H4C FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TFIIB-like ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5702 Trypanosoma brucei brucei])</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3h4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3h4c RCSB], [http://www.ebi.ac.uk/pdbsum/3h4c PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h4c OCA], [https://pdbe.org/3h4c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h4c RCSB], [https://www.ebi.ac.uk/pdbsum/3h4c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h4c ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q257B3_TRYBB Q257B3_TRYBB]
In trypanosomes, the production of mRNA relies on the synthesis of the spliced leader (SL) RNA. Expression of the SL RNA is initiated at the only known RNA polymerase II promoter in these parasites. In the pathogenic trypanosome, Trypanosoma brucei, transcription factor IIB (tTFIIB) is essential for SL RNA gene transcription and cell viability, but has a highly divergent primary sequence in comparison to TFIIB in well-studied eukaryotes. Here we describe the 2.3 A resolution structure of the C-terminal domain of tTFIIB (tTFIIB(C)). The tTFIIB(C) structure consists of 2 closely packed helical modules followed by a C-terminal extension of 32 aa. Using the structure as a guide, alanine substitutions of basic residues in regions analogous to functionally important regions of the well-studied eukaryotic TFIIB support conservation of a general mechanism of TFIIB function in eukaryotes. Strikingly, tTFIIB(C) contains additional loops and helices, and, in contrast to the highly basic DNA binding surface of human TFIIB, contains a neutral surface in the corresponding region. These attributes probably mediate trypanosome-specific interactions and have implications for the apparent bidirectional transcription by RNA polymerase II in protein-encoding gene expression in these organisms.
 
Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei.,Ibrahim BS, Kanneganti N, Rieckhof GE, Das A, Laurents DV, Palenchar JB, Bellofatto V, Wah DA Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13242-7. Epub 2009 Aug 3. PMID:19666603<ref>PMID:19666603</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Trypanosoma brucei brucei]]
[[Category: Trypanosoma brucei brucei]]
[[Category: Bellofatto, V]]
[[Category: Bellofatto V]]
[[Category: Das, A]]
[[Category: Das A]]
[[Category: Ibrahim, B Syed]]
[[Category: Kanneganti N]]
[[Category: Kanneganti, N]]
[[Category: Laurents DV]]
[[Category: Laurents, D V]]
[[Category: Palenchar JB]]
[[Category: Palenchar, J B]]
[[Category: Rieckhof GE]]
[[Category: Rieckhof, G E]]
[[Category: Syed Ibrahim B]]
[[Category: Wah, D A]]
[[Category: Wah DA]]
[[Category: Cyclin]]
[[Category: Transcription]]
[[Category: Transcription factor tfiib repeat]]

Latest revision as of 09:57, 21 February 2024

Structure of the C-terminal Domain of Transcription Factor IIB from Trypanosoma brucei

3h4c, resolution 2.30Å

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