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==Computationally designed TIM-barrel protein, HalfFLR==
==Computationally designed TIM-barrel protein, HalfFLR==
<StructureSection load='3tdm' size='340' side='right' caption='[[3tdm]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
<StructureSection load='3tdm' size='340' side='right'caption='[[3tdm]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3tdm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TDM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TDM FirstGlance]. <br>
<table><tr><td colspan='2'>[[3tdm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TDM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TDM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tdn|3tdn]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tdm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tdm RCSB], [http://www.ebi.ac.uk/pdbsum/3tdm PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tdm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tdm OCA], [https://pdbe.org/3tdm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tdm RCSB], [https://www.ebi.ac.uk/pdbsum/3tdm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tdm ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
It has been demonstrated previously that symmetric, homodimeric proteins are energetically favored, which explains their abundance in nature. It has been proposed that such symmetric homodimers underwent gene duplication and fusion to evolve into protein topologies that have a symmetric arrangement of secondary structure elements-"symmetric superfolds". Here, the ROSETTA protein design software was used to computationally engineer a perfectly symmetric variant of imidazole glycerol phosphate synthase and its corresponding symmetric homodimer. The new protein, termed FLR, adopts the symmetric (betaalpha)(8) TIM-barrel superfold. The protein is soluble and monomeric and exhibits two-fold symmetry not only in the arrangement of secondary structure elements but also in sequence and at atomic detail, as verified by crystallography. When cut in half, FLR dimerizes readily to form the symmetric homodimer. The successful computational design of FLR demonstrates progress in our understanding of the underlying principles of protein stability and presents an attractive strategy for the in silico construction of larger protein domains from smaller pieces.
Exploring symmetry as an avenue to the computational design of large protein domains.,Fortenberry C, Bowman EA, Proffitt W, Dorr B, Combs S, Harp J, Mizoue L, Meiler J J Am Chem Soc. 2011 Nov 16;133(45):18026-9. Epub 2011 Oct 21. PMID:21978247<ref>PMID:21978247</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Synthetic construct]]
[[Category: Bowman, E]]
[[Category: Bowman E]]
[[Category: Dorr, B]]
[[Category: Dorr B]]
[[Category: Fortenberry, C]]
[[Category: Fortenberry C]]
[[Category: Harp, J M]]
[[Category: Harp JM]]
[[Category: Mizoue, L]]
[[Category: Mizoue L]]
[[Category: Profitt, W]]
[[Category: Profitt W]]
[[Category: De novo protein]]
[[Category: Symmetric superfold]]
[[Category: Tim-barrel]]
Retrieved from "https://proteopedia.org/3tdm"