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==The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to 6-bromo-6-deoxy-D-glucose==
==The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to 6-bromo-6-deoxy-D-glucose==
<StructureSection load='4gc0' size='340' side='right' caption='[[4gc0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='4gc0' size='340' side='right'caption='[[4gc0]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4gc0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GC0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4gc0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GC0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=6BG:6-BROMO-6-DEOXY-BETA-D-GLUCOPYRANOSE'>6BG</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gby|4gby]], [[4gbz|4gbz]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6BG:6-BROMO-6-DEOXY-BETA-D-GLUCOPYRANOSE'>6BG</scene>, <scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">xylE, b4031, JW3991 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gc0 OCA], [https://pdbe.org/4gc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gc0 RCSB], [https://www.ebi.ac.uk/pdbsum/4gc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gc0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gc0 RCSB], [http://www.ebi.ac.uk/pdbsum/4gc0 PDBsum]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/XYLE_ECOLI XYLE_ECOLI] Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).
Glucose transporters are essential for metabolism of glucose in cells of diverse organisms from microbes to humans, exemplified by the disease-related human proteins GLUT1, 2, 3 and 4. Despite rigorous efforts, the structural information for GLUT1-4 or their homologues remains largely unknown. Here we report three related crystal structures of XylE, an Escherichia coli homologue of GLUT1-4, in complex with d-xylose, d-glucose and 6-bromo-6-deoxy-D-glucose, at resolutions of 2.8, 2.9 and 2.6 A, respectively. The structure consists of a typical major facilitator superfamily fold of 12 transmembrane segments and a unique intracellular four-helix domain. XylE was captured in an outward-facing, partly occluded conformation. Most of the important amino acids responsible for recognition of D-xylose or d-glucose are invariant in GLUT1-4, suggesting functional and mechanistic conservations. Structure-based modelling of GLUT1-4 allows mapping and interpretation of disease-related mutations. The structural and biochemical information reported here constitutes an important framework for mechanistic understanding of glucose transporters and sugar porters in general.
 
Crystal structure of a bacterial homologue of glucose transporters GLUT1-4.,Sun L, Zeng X, Yan C, Sun X, Gong X, Rao Y, Yan N Nature. 2012 Oct 18;490(7420):361-6. doi: 10.1038/nature11524. PMID:23075985<ref>PMID:23075985</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Symporter 3D structures|Symporter 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli k-12]]
[[Category: Escherichia coli K-12]]
[[Category: Sun, L F]]
[[Category: Large Structures]]
[[Category: Yan, C Y]]
[[Category: Sun LF]]
[[Category: Yan, N]]
[[Category: Yan CY]]
[[Category: Zeng, X]]
[[Category: Yan N]]
[[Category: D-xylose:proton symporter]]
[[Category: Zeng X]]
[[Category: Mf]]
[[Category: Transport protein]]

Latest revision as of 15:43, 14 March 2024

The structure of the MFS (major facilitator superfamily) proton:xylose symporter XylE bound to 6-bromo-6-deoxy-D-glucose

4gc0, resolution 2.60Å

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