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[[Image:2h8a.jpg|left|200px]]


{{Structure
==Structure of Microsomal Glutathione Transferase 1 in Complex with Glutathione==
|PDB= 2h8a |SIZE=350|CAPTION= <scene name='initialview01'>2h8a</scene>, resolution 3.20&Aring;
<StructureSection load='2h8a' size='340' side='right'caption='[[2h8a]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=GTT:GLUTATHIONE'>GTT</scene>
<table><tr><td colspan='2'>[[2h8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H8A FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8a OCA], [https://pdbe.org/2h8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h8a RCSB], [https://www.ebi.ac.uk/pdbsum/2h8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h8a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MGST1_RAT MGST1_RAT] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/2h8a_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2h8a ConSurf].
<div style="clear:both"></div>


'''Structure of Microsomal Glutathione Transferase 1 in Complex with Glutathione'''
==See Also==
 
*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
Synthesis of mediators of fever, pain and inflammation as well as protection against reactive molecules and oxidative stress is a hallmark of the MAPEG superfamily (membrane associated proteins in eicosanoid and glutathione metabolism). The structure of a MAPEG member, rat microsomal glutathione transferase 1, at 3.2 A resolution, solved here in complex with glutathione by electron crystallography, defines the active site location and a cytosolic domain involved in enzyme activation. The glutathione binding site is found to be different from that of the canonical soluble glutathione transferases. The architecture of the homotrimer supports a catalytic mechanism involving subunit interactions and reveals both cytosolic and membraneous substrate entry sites, providing a rationale for the membrane location of the enzyme.
[[Category: Large Structures]]
 
==About this Structure==
2H8A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H8A OCA].
 
==Reference==
Structural basis for detoxification and oxidative stress protection in membranes., Holm PJ, Bhakat P, Jegerschold C, Gyobu N, Mitsuoka K, Fujiyoshi Y, Morgenstern R, Hebert H, J Mol Biol. 2006 Jul 28;360(5):934-45. Epub 2006 Jun 5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16806268 16806268]
[[Category: Glutathione transferase]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Hebert H]]
[[Category: Hebert, H.]]
[[Category: GTT]]
[[Category: membrane protein]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:13:55 2008''

Latest revision as of 09:31, 14 February 2024

Structure of Microsomal Glutathione Transferase 1 in Complex with Glutathione

2h8a, resolution 3.20Å

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